您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

CRISPR/Cas9 mediated knockout of laccase2 impairs cuticle tanning and pigmentation in the tobacco cutworm, Spodoptera litura

文献类型： 外文期刊

作者： Peng, Yingchuan 1 ; He, Yu 2 ; Ling, Xinyi 1 ; Zeng, Lin 1 ; Cao, Lixia 1 ; Xie, Lexin 1 ; Ma, Long 3 ; Zhang, Wanna 1 ; Jia, Zhongqiang 4 ;

作者机构： 1.Jiangxi Agr Univ, Inst Entomol, Nanchang 330045, Peoples R China

2.Nanjing Agr Univ, Coll Plant Protect, Key Lab Integrated Pest Management Crops East Chin, Minist Agr, Nanjing 210095, Peoples R China

3.Jiangxi Sci & Technol Normal Univ, Coll Life Sci, Nanchang 330013, Peoples R China

4.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr, Key Lab Synthet Biol,Minist Agr & Rural Affairs,Sh, Shenzhen 518120, Peoples R China

关键词： Spodoptera litura; Laccase; Pigmentation; Cuticle tanning; CRISPR/Cas9

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN： 0048-3575

年卷期： 2025 年 212 卷

页码：

收录情况： SCI

摘要： Cuticle tanning is a vital physiological process in insects, mediated by the phenol oxidase Laccase2 (Lac2). To elucidate the functional role underlying cuticle formation in the economically important pest Spodoptera litura, SlitLac2 was functionally characterized in this study. The identified SlitLac2 encodes a protein containing conserved Cu-oxidase domains and a conserved cysteine-rich region, exhibiting high sequence simility to other lepidopteran Lac2 orthologs. Quantitative expression analysis revealed peak expression of SlitLac2 in 3rd instar larvae, with 251.45-, 25.06-, 34.43-, 341.04-, 31.67-, 158.04-, 5.68-, 228.43- and 668.31-fold higher expression levels than those in eggs, 1st, 2nd, 4th, 5th, 6th larvae, pupae, male adults and female adults, respectively. While tissue-specific profiling showed predominant expression in the epidermis, displaying 678.28-, 6.18-, 51.35-, 28.21- and 176.59-fold expression levels, compared to those in the hemolymph, head, malpighian tubule, fat body and midgut, respectively. Knockout of SlitLac2 severely impaired normal cuticle tanning of the 3rd, 4th, 5th, 6th instar larvae and pupae, ultimately leading to failure of adult eclosion. This study provides the first functional characterization of SlitLac2 in S. litura, establishing its critical role in cuticle formation and offering new insights into the molecular basis of insect development. Our findings identify SlitLac2 as a potential target for developing novel pest control strategies against this agriculturally significant species.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台