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Development and identification of glyphosate-tolerant transgenic soybean via direct selection with glyphosate

文献类型： 外文期刊

作者： Guo Bing-fu 1 ; Hong Hui-long 1 ; Han Jia-nan 1 ; Zhang Li-juan 1 ; Liu Zhang-xiong 1 ; Guo Yong 1 ; Qiu Li-juan 1 ;

作者机构： 1.Chinese Acad Agr Sci, Natl Key Facil Crop Gene Resources & Genet Improv, MOA Key Lab Soybean Biol, Inst Crop Sci, Beijing 100081, Peoples R China

2.Jiangxi Acad Agr Sci, Jiangxi Prov Key Lab Oilcrops Biol, Crops Res Inst, Nanchang 330200, Jiangxi, Peoples R China

关键词： soybean; transformation; spot assay; glyphosate selection; transgenic plants

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2020 年 19 卷 5 期

页码：

收录情况： SCI

摘要： Glyphosate-tolerant soybean is the most widely planted genetically modified crop worldwide. However, soybean remains recalcitrant to routine transformation because of the low infection efficiency of Agrobacterium to soybean and lack of useful selectable markers. In this study, several Agrobacterium strains and cell densities were compared by transient expression of the GUS gene. The results showed that Agrobacterium strain Ag10 at cell densities of OD600 of 0.6-0.9 yielded the highest infection efficiency in Agrobacterium-mediated soybean cotyledonary node transformation system. Meanwhile, a simple and rapid method was developed for identification of glyphosate tolerance in putative T-0 transgenic plants, consisting of spotting plantlets with 1 mu L Roundup (R). The whole cycle of genetic transformation could be shortened to about 3 mon by highly efficient selection with glyphosate during the transformation process and application of the spot assay in putative T-0 transgenic plantlets. The transformation frequency ranged from 2.9 to 5.6%. This study provides an improved protocol for development and identification of glyphosate-tolerant transgenic soybeans.

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