文献类型： 外文期刊

作者： Gao, Y. ¹ ; Zeng, X. D. ¹ ; Ren, B. ¹ ; Zeng, J. R. ¹ ; Xu, T. ¹ ; Yang, Y. Z. ² ; Hu, X. C. ² ; Zhu, Z. Y. ¹ ; Shi, L. M. ³ ;

作者机构： 1.Hunan Univ, Coll Biol, Hunan Prov Key Lab Plant Functional Genom & Dev R, Changsha 410008, Hunan, Peoples R China

2.Yahua Seeds Sci Acad Hunan, Changsha 410119, Hunan, Peoples R China

3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100081, Peoples R China

4.Cent South Univ Forestry & Technol, Hunan Prov Key Lab Control Forest Dis & Pests, Changsha 410008, Hunan, Peoples R China

5.Hunan Agr Univ, Hunan Prov Key Lab Biol & Control Plant Dis & Ins, Changsha 410128, Hunan, Peoples R China

关键词： active metabolites; biological control; induced systemic resistance; Magnaporthe oryzae; rice endophyte; Streptomyces albidoflavus

期刊名称：PLANT PATHOLOGY （ 影响因子：2.59； 五年影响因子：2.924 ）

ISSN： 0032-0862

年卷期： 2020 年 69 卷 2 期

页码：

收录情况： SCI

摘要： Rice blast, caused by the fungus Magnaporthe oryzae, can result in notable yield losses in rice production. The objective of this study was to investigate the potential of a rice endophytic isolate, Streptomyces albidoflavus OsiLf-2, on the control of rice blast and the possible mechanisms involved. In vitro assays displayed a variety of antagonistic effects of OsiLf-2 against different physiological races of M. oryzae, with peak mycelial growth inhibition ranging from 74.1% to 83.0%. In vivo tests of OsiLf-2 showed 18.0% and 19.6% reduction in disease index in greenhouse and field conditions, respectively. The stable active metabolites in its cell-free culture filtrate inhibited the mycelial growth, spore germination and appressorial formation of M. oryzae in a dose-dependent manner. They also possessed strong antifungal capacities toward various phytopathogens in vitro. OsiLf-2 secreted multiple antimicrobial compounds, cell wall degradation enzymes, siderophore, plant hormones, and 1-aminocyclopropane-1-carboxylate deaminase, which might function in direct or indirect resistance to M. oryzae. In addition, a variety of defence responses were induced in OsiLf-2-treated rice, including hydrogen peroxide (H2O2) accumulation, callose deposition, defence-related enzymes activation, and elevated expression of salicylic acid (SA) and jasmonic acid (JA) pathways genes, which might contribute in resisting pathogen attack. The significant biological control activity and host defence-stimulation ability of OsiLf-2 suggest that this endophytic actinobacterial strain could be a promising candidate in the management of rice blast disease.