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Differential responses of root growth to nutrition with different ammonium/nitrate ratios involve auxin distribution in two tobacco cultivars

文献类型： 外文期刊

作者： Meng Lin 1 ; Dong Jian-xin 1 ; Wang Shu-sheng 1 ; Song Ke 1 ; Ling Ai-fen 2 ; Yang Jin-guang 1 ; Xiao Zhi-xin 3 ; Li Wei; 1 ;

作者机构： 1.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Tobacco Biol & Proc, Tobacco Res Inst, Qingdao 266101, Shandong, Peoples R China

2.Sichuan Tobacco Corp, Liangshan Branch, Xichang 615000, Peoples R China

3.Yunnan Tobacco Co, Baoshan Branch, Baoshan 678000, Peoples R China

4.Hongyunhonghe Tobacco Grp Co Ltd, Kunming 650231, Yunnan, Peoples R China

关键词： auxin; lateral root; formation and elongation; tobacco; NH4+/NO3- ratio

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2019 年 18 卷 12 期

页码：

收录情况： SCI

摘要： Nitrogen (N), the major forms of which are nitrate (NO3-) and ammonium (NH4+), plays an important role in plant growth and mediation of root development. However, the role of auxin in root growth in response to different NH4+/NO3- ratios remains unclear. Two tobacco cultivars (Nicotiana tabacum L.) were adopted in this study, which displayed variant growth features under the situations with sole NO3- nutrition ratio (NH4+/NO3- ratio: 0/100), low NO3- nutrition ratio (NH4+/NO3- ratio: 97/3), and optimal NH4+/NO3- ratio (50/50). We investigated the effects of the different NH4+/NO3- ratios on the formation and elongation of lateral roots (LRs), auxin concentration, DR5::GUS expression, H-3-labeled indole acetic acid ([H-3]IAA) transport, and the expression of six PIN genes in tobacco roots. We also examined the effects of exogenous auxin and a transport inhibitor on LRs growth. The results are shown as follows, compared to optimal N nutrition conditions, the biomass and nitrogen (N) accumulation were largely reduced by sole and low NO3- nutrition treatment in NC89, but no difference was observed in Zhongyan 100. In most cases, sole and low NO3- nutrition impaired the elongation and formation of first-order lateral roots (1 degrees LRs), only in NC89, thus reducing the root growth. IAA concentration and DR5::GUS expression levels decreased in roots when NC89 was subjected to sole and low NO3- nutrition media, suggesting that different NH4+/NO3- ratios affect the transport of auxin from leaves to roots. Results were similar following exogenous NAA application to low NO3- nutrition treated seedlings. Based on direct [H-3]IAA transport measurement, the transport of polar auxin from shoots to roots decreased due to low NO3- nutrition. PIN4 expression levels were markedly decreased in roots of NC89 by sole and low NO3- nutrition, while they were unaffected in Zhongyan 100 roots. Overall, our findings suggest that LRs formation in tobacco seedlings is regulated by NH4+/NO3- ratios via modifying polar transport of auxin.

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