文献类型： 外文期刊

作者： Liang, Ying ¹ ; Dong, Yating ³ ; Yang, Qichang ² ; Urano, Daisuke ³ ; Wang, Zheng ² ;

作者机构： 1.Sichuan Acad Agr Sci, Hort Res Inst, Vegetable Germplasm Innovat & Variety Improvement, Chengdu 610066, Peoples R China

2.Chinese Acad Agr Sci, Inst Urban Agr, Chengdu 610213, Peoples R China

3.Natl Univ Singapore, Temasek Life Sci Lab, Singapore 117604, Singapore

4.Natl Univ Singapore, Dept Biol Sci, Singapore 117604, Singapore

关键词： Lettuce; Light quality; Nitrate deficiency; Anthocyanins; Flavonoids; Indoor farming

期刊名称：ENVIRONMENTAL AND EXPERIMENTAL BOTANY （ 影响因子：5.7； 五年影响因子：6.0 ）

ISSN： 0098-8472

年卷期： 2023 年 213 卷

页码：

收录情况： SCI

摘要： Light quality and nutrient parameters alter plant growth and metabolic pathways in leafy greens, therefore being primarily optimized in indoor precision agriculture. While their individual effects were studied extensively with large-scale phenomics and transcriptomics, the combinatorial effects remain poorly understood. Here, we explored the hidden interactions among light-and nitrate-responsive transcriptomes and their associations with six growth and biochemical traits, using the full factorial experimental design with three lights (red (R), blue (B) and a 1:1 mixture of red and blue (RB)), two nitrate concentrations and two cultivars (Green oak-leaf and Red oak-leaf). Multifactor analysis of 36 RNA-sequencing data revealed their largely independent but additive effects that are associated with MYB, bHLH and GATA family transcription factors. Red light and sufficient nitrate supply additively increased the primary metabolite pathway and bHLH and GATA genes, leading to a high growth rate. Blue and RB lights, together with nitrate deficiency, induced flavonoid pathway and MYB genes. Variety-specific deviations were detected in late anthocyanin biosynthetic genes and specific MYB transcription factors that would explain anthocyanin accumulations in the Red oak-leaf cultivar. Taken together, our large-scale transcriptomic and physiologic results suggest that the two environmental parameters (i.e., light quality and nitrate level) (1) utilize independent transcriptional regulatory mechanisms to achieve similar phenotypic and metabolic changes, and (2) induce the variety-specific anthocyanin accumulations via a small number of genes that show different gene expression profiles between the two cultivars.