Unlocking dual-mode enzyme activities on bacterial surface: Directional recognition and swift capture of Alicyclobacillus acidoterrestris from fruit juices
文献类型： 外文期刊
作者： Li, Bingzhi 1 ; Cheng, Kun 1 ; Chen, Pengyu 1 ; Luo, Linpin 1 ; Zhang, Liang 1 ; Du, Ting 1 ; Zhang, Tong 1 ; Li, Qingqing 1 ; Ma, Yiyue 1 ; Sun, Jing 2 ; Jin, Maojun 4 ; Wang, Jianlong 1 ; Chen, Yiping 3 ;
作者机构： 1.Northwest A&F Univ, Coll Food Sci & Engn, 22 Xinong Rd, Yangling 712100, Shaanxi, Peoples R China
2.Chinese Acad Sci, Northwest Inst Plateau Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resourc, 23 Xinning Rd, Xining 810008, Qinghai, Peoples R China
3.Dalian Polytech Univ, State Key Lab Marine Food Proc & Safety Control, Dalian 116034, Liaoning, Peoples R China
4.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
关键词： Alicyclobacillus acidoterrestris; Carbon-Lysozyme nanohybrids; Bacterial adsorption; Magnetic separation
期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.0； 五年影响因子：8.5 ）
ISSN： 0963-9969
年卷期： 2025 年 200 卷
页码：
收录情况： SCI
摘要： The acidophilic and heat-resistant characteristics of Alicyclobacillus acidoterrestris (A. acidoterrestris) pose significant challenges to fruit juice production. Traditional thermal removal methods are often ineffective against this resilient bacterium. To address this issue, we developed a novel adsorbent, magnetic carbonation carbonlysozyme nanohybrid (MCL), composed of magnetic nanoparticles with a thin carbon shell and covalently grafted lysozyme. The outer lysozyme facilitates binding to the bacterial surface through two modes: electrostatic attraction and chemical interaction, acting as a vital engine for bacterial adhesion. The ultrathin carbon coating enhances dispersion, reduces magnetic loss, provides more adsorption sites for lysozyme grafting, and ensures stable function in acidic environments. Benefiting from the large surface area of MCL and the specific peptidoglycan recognition structure of lysozyme, MCL exhibits rapid adsorption kinetics and can completely remove 104 CFU/mL of A. acidoterrestris from juice within 20 min. The MCL demonstrates excellent capture performance, negligible cytotoxicity, and no significant impact on juice quality, offering a promising non-thermal strategy to improve juice safety.
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