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Bacterial ghosts engineered with lipidated antigens as an adjuvant-free vaccine for Chlamydia abortus

文献类型： 外文期刊

作者： Zhang, Huaiyu 1 ; Li, Wei 1 ; Li, Yunhui 2 ; Wang, Yihan 1 ; Jin, Youshun 1 ; Tong, Dewen 2 ; Li, Zhaocai 1 ; Zhou, Jizhang 1 ;

作者机构： 1.Lanzhou Univ, Lanzhou Vet Res Inst, Chinese Acad Agr Sci, Coll Vet Med,State Key Lab Anim Dis Control & Prev, Lanzhou 730000, Peoples R China

2.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Peoples R China

3.Gansu Prov Res Ctr Basic Disciplines Pathogen Biol, Lanzhou 730046, Peoples R China

关键词： Bacterial ghost; Vaccine; lpp'-ompA; Lipoprotein; Chlamydia abortus

期刊名称：INTERNATIONAL JOURNAL OF PHARMACEUTICS （ 影响因子：5.2； 五年影响因子：5.7 ）

ISSN： 0378-5173

年卷期： 2024 年 666 卷

页码：

收录情况： SCI

摘要： Bacterial ghosts (BGs) provide novel vaccine delivery platforms because of their inherent adjuvant properties and efficient antigen delivery capabilities. However, effective engineering strategies are required to modify them for different antigens. In this study, the Escherichia coli (E. E. coli) ) ghost was modified by using a lpp'-ompA chimera, a widely used bacterial surface display vector, with a protective antigen macrophage infectivity potentiator (MIP) of Chlamydia abortus (C. C. abortus), ), and its protective effect was evaluated in a mouse model. The MIP fusion protein accumulated at 1.2% of the ghost total protein mass and a significant portion of the protein was modified into lipoproteins upon translocation to the BG surface. Lipidated MIP-modified recombinant E. coli ghosts (rECGlpp'-MIP) effectively promoted antigen-presenting cells (APCs) uptake of antigens and stimulated APCs activation in vivo and in vitro. . Immunization with rECG-lpp'-MIP and no adjuvant induced intense specific humoral responses as well as Th1-biased cellular immune responses, which significantly improved the efficiency of C. abortus infection clearance in mice and reduced pathological damage to the uterus. In summary, this study demonstrates that recombinant E. coli ghosts modified with lipidated antigens could help to develop an effective C. abortus vaccine and aid in the development of a universal adjuvant-free vaccine platform.

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