Development and identification of molecular markers of GhHSP70-26 related to heat tolerance in cotton
文献类型： 外文期刊
作者： Guo, Yaping 1 ; Chen, Qin 2 ; Qu, Yanying 2 ; Deng, Xiaojuan 2 ; Zheng, Kai 2 ; Wang, Ning 1 ; Shi, Jianbin 1 ; Zhang, Yinbin 1 ; Chen, Quanjia 2 ; Yan, Gentu 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang, Peoples R China
2.Xinjiang Agr Univ, Coll Agron, Urumqi, Peoples R China
关键词： Heat stress; Promoter; Functional markers; KASP; Cotton
期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）
ISSN： 0378-1119
年卷期： 2023 年 874 卷
页码：
收录情况： SCI
摘要： Heat stress significantly affect plant growth and development, which is an important factor contributing to crop yield loss. However, heat shock proteins (HSPs) in plants can effectively alleviate cell damage caused by heat stress. In order to rapidly and accurately cultivate heat-tolerant cotton varieties, this study conducted correlation analysis between heat tolerance index and insertion/deletion (In/Del) sites of GhHSP70-26 promoter in 39 cotton materials, so as to find markers related to heat tolerance function of cotton, which can be used in molecular marker-assisted breeding. The results showed the natural variation allele (Del22 bp) type at-1590 bp upstream of GhHSP70-26 promoter (haplotype2, Hap2) in cotton (Gossypium spp.) promoted GhHSP70-26 expression under heat stress. The relative expression level of GhHSP70-26 of M-1590- Del22 cotton materials were significantly higher than that of M-1590-In type cotton materials under heat stress (40 celcius). Also, M-1590- Del22 material had lower conductivity and less cell damage after heat stress, indicating that it is a heat resistant cotton material. The Hap1 (M-1590-In) promoter was mutated into Hap1del22, and Hap1 and Hap1del22 were fused with GUS to transform Arabidopsis thaliana. Furthermore, Hap1del22 promoter had higher induction activity than Hap1 under heat stress and abscisic acid (ABA) treatment in transgenic Arabidopsis thaliana. Further analysis confirmed that M-1590- Del22 was the dominant heat-resistant allele. In summary, these results identify a key and previously unknown natural variation in GhHSP70-26 with respect to heat tolerance, providing a valuable functional mo-lecular marker for genetic breeding of cotton and other crops with heat tolerance.
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