EXPRESSION OF THE BARLEY YELLOW DWARF VIRUS-GAV MOVEMENT PROTEIN AND ITS DETECTION IN THE INFECTED AND TRANSGENIC PLANTS
文献类型: 外文期刊
作者: Xie, J. 1 ; Wang, X. 1 ; Liu, Y. 1 ; Peng, Y. 1 ; Zhou, G. 1 ;
作者机构: 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100094, Peoples R China
关键词: Barley yellow dwarf virus-GAV;movement protein;expression;immunization
期刊名称:ACTA VIROLOGICA ( 影响因子:1.162; 五年影响因子:1.255 )
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收录情况: SCI
摘要: Movement proteins (MPs) that facilitate virus movement in the plants were identified in a number of plant viruses. In this study, full-length MP gene of the Chinese isolate Barley yellow dwarf virus-GAV (BYDV-GAV) was cloned and expressed in Escherichia coll. About 32% of the expressed MP was soluble providing the concentration of isopropyl-B-D-galactopyranoside (IPTG), time of the induction, temperature and shaking speed were optimized. The soluble MP was purified using nickel-affinity column. Immune serum prepared against purified MP was used for the detection of MP in the BYDV-GAV infected leaves of oat and in the leaves of transgenic wheat plants expressing the full-length and truncated MP gene.
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