文献类型： 外文期刊

作者： Li, Pengtao ¹ ; Chen, Yu ¹ ; Yang, Rui ² ; Sun, Zhihao ² ; Ge, Qun ² ; Xiao, Xianghui ¹ ; Yang, Shuhan ¹ ; Li, Yanfang ¹ ; Liu, Qiankun ¹ ; Zhang, Aiming ¹ ; Xing, Baoguang ¹ ; Wu, Bei ¹ ; Du, Xue ¹ ; Liu, Xiaoyan ¹ ; Tang, Baomeng ¹ ; Gong, Juwu ² ; Lu, Quanwei ² ; Shi, Yuzhen ² ; Yuan, Youlu ² ; Peng, Renhai ¹ ; Shang, Haihong ³ ;

作者机构： 1.Anyang Inst Technol, Sch Biotechnol & Food Engn, Anyang 455000, Peoples R China

2.Chinese Acad Agr Sci, Inst Cotton Res, Natl Key Lab Cotton Biobreeding & Integrated Utili, Anyang 455000, Peoples R China

3.Zhengzhou Univ, Natl Key Lab Cotton Biobreeding & Integrated Utili, Zhengzhou 450001, Peoples R China

4.Xinjiang Prod & Construction Corps, Agr Res Inst, Div 7, Kuitun 833200, Peoples R China

5.Agr Technol Popularizat Ctr Kashgar, Kashgar 844000, Peoples R China

关键词： cotton chromosome segment substitution lines; fiber quality; weighted gene co-expressed network analyses; functional enrichment analysis; introgressive gene identification

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2024 年 13 卷 16 期

页码：

收录情况： SCI

摘要： Fiber length (FL) and strength (FS) are the core indicators for evaluating cotton fiber quality. The corresponding stages of fiber elongation and secondary wall thickening are of great significance in determining FL and FS formation, respectively. QTL mapping and high-throughput sequencing technology have been applied to dissect the molecular mechanism of fiber development. In this study, 15 cotton chromosome segment substitution lines (CSSLs) with significant differences in FL and FS, together with their recurrent parental Gossypium hirsutum line CCRI45 and donor parent G. barbadense line Hai1, were chosen to conduct RNA-seq on developing fiber samples at 10 days post anthesis (DPA) and 20 DPA. Differentially expressed genes (DEGs) were obtained via pairwise comparisons among all 24 samples (each one with three biological repeats). A total of 969 DEGs related to FL-high, 1285 DEGs to FS-high, and 997 DEGs to FQ-high were identified. The functional enrichment analyses of them indicated that the GO terms of cell wall structure and ROS, carbohydrate, and phenylpropanoid metabolism were significantly enriched, while the GO terms of glucose and polysaccharide biosynthesis, and brassinosteroid and glycosylphosphatidylinositol metabolism could make great contributions to FL and FS formation, respectively. Weighted gene co-expressed network analyses (WGCNA) were separately conducted for analyzing FL and FS traits, and their corresponding hub DEGs were screened in significantly correlated expression modules, such as EXPA8, XTH, and HMA in the fiber elongation and WRKY, TDT, and RAC-like 2 during secondary wall thickening. An integrated analysis of these hub DEGs with previous QTL identification results successfully identified a total of 33 candidate introgressive DEGs with non-synonymous mutations between the Gh and Gb species. A common DEG encoding receptor-like protein kinase 1 was reported to likely participate in fiber secondary cell thickening regulation by brassionsteroid signaling. Such valuable information was conducive to enlightening the developing mechanism of cotton fiber and also provided an abundant gene pool for further molecular breeding.