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Bmcas-1 plays an important role in response against BmNPV infection in vitro

文献类型： 外文期刊

作者： Wang, Xin 1 ; Zhao, Zi-qin 1 ; Huang, Xin-ming 1 ; Ding, Xin-yi 1 ; Zhao, Chun-xiao 1 ; Li, Mu-wang 1 ; Wu, Yang-chun; 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Jiangsu Key Lab Sericultural Biol & Biotechnol, Zhenjiang 212100, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Key Lab Silkworm & Mulberry Genet Improvement, Sericultural Res Inst, Zhenjiang, Jiangsu, Peoples R China

3.Yancheng Teachers Univ, Jiangsu Key Lab Bioresources Saline Soils, Jiangsu Synthet Innovat Ctr Coastal Bioagr, Sch Wetland,Jiangsu Prov Key Lab Coastal Wetland, Yancheng 224007, Jiangsu, Peoples R China

关键词： apoptosis pathway; Bmcaspase‐ 1; BmNPV; Bombyx mori; response mechanism

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：1.536； 五年影响因子：1.546 ）

ISSN： 0739-4462

年卷期：

页码：

收录情况： SCI

摘要： Apoptosis, as one kind of innate immune system, is involved in host response against pathogens innovation. Caspases play a vital role in the execution stage of host cell apoptosis. It has been reported that Bmcaspase-1 (Bmcas-1) has a close relationship with Bombyx mori nucleopolyhedrovirus (BmNPV) infection for its differentially expressed patterns after viral infection. However, its underlying response mechanism is still unclear. The significant differential expression of Bmcas-1 in different tissues of differentially resistant strains revealed its vital role in BmNPV infection. To further validate its role in BmNPV infection, budded virus (BV)-eGFP was analyzed after knockdown and overexpression of Bmcas-1 by small interfering RNA and the pIZT-mCherry vector, respectively. The reproduction of BV-eGFP obviously increased at 72 h after knockdown of Bmcas-1, and decreased after overexpression in BmN cells. Moreover, the conserved functional domain of Cas-1 among different species and the closed evolutionary relationship of Cas-1 in Lepidoptera hinted that Bmcas-1 might be associated with apoptosis, and this was also validated by the apoptosis inducer, Silvestrol, and the inhibitor, Z-DEVD-FMK. Therefore, Bmcas-1 plays an essential antiviral role by activating apoptosis, and this result lays a fundament for clarifying the molecular mechanism of silkworm in response against BmNPV infection and breeding of resistant strains.

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