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Cloning, expression, and functional analysis of the beta-1,3-glucanase gene in Ostrinia furnacalis

文献类型： 外文期刊

作者： Liu, Danmei 1 ; Wang, Rui 2 ; Yang, Jun 2 ; Yang, Qing 3 ;

作者机构： 1.Eastern Liaoning Univ, Coll Agr, Dandong, Peoples R China

2.Dalian Univ Technol, State Key Lab Biol Plant Dis & Insect Pests, Sch Bioengn, Dalian 116024, Peoples R China

3.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

4.Chinese Acad Agr Sci, Shenzhen Agr Genome Res Inst, Beijing 100193, Peoples R China

关键词： functional analysis; gene cloning; Ostrinia furnacalis; beta-1,3-glucanase

期刊名称：BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY （ 影响因子：1.638； 五年影响因子：1.591 ）

ISSN： 0885-4513

年卷期：

页码：

收录情况： SCI

摘要： The beta-1,3-glucanase gene in Ostrinia furnacalis was first obtained by RT-PcR. The real-time fluorescence quantitative PCR showed that the expression level of beta-1,3-glucanase in the midgut of O. furnacalis was higher than in other tissues. Moreover, the expression level in the larval stage was higher in egg, pupa, and adult stages. The optimal pH of recombinant O. furnacalis beta-1,3-glucanase OfLam to the substrate laminarin was 4.5, and the optimum reaction temperature was 50 degrees C. The enzyme exhibited a K-M of 1.59 +/- 0.28 mg/mL and a k(cat) of 15.8 +/- 0.66 s(-)(1). Ostrinia furnacalis beta-1,3-glucanase has a similar catalytic efficiency to other insect-derived beta-1,3-glucanases. The recombinant OfLam has a broad substrate spectrum and can hydrolyze fungal cell walls, suggesting a new source of enzymes for biological control strategies that target fungal cell walls.

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