您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effects of a Protease Inhibitor Protein on Buchnera aphidicola and Gene Expression in Pea Aphids (Acyrthosiphon pisum)

文献类型： 外文期刊

作者： Zhang, Tingting 1 ; Li, Qiannan 2 ; Zheng, Li 3 ; Mao, Jianjun 2 ; Zeng, Fanrong 2 ;

作者机构： 1.Fuzhou Univ, Sch Adv Mfg, Jinjiang, Peoples R China

2.Chinese Acad Agr Sci, Inst Plant Protect, Key Lab Integrated Pest Management Crops, Minist Agr, Beijing, Peoples R China

3.Minist Nat Resources, Inst Oceanog 1, Key Lab Marine Ecoenvironm Sci & Technol, Qingdao, Peoples R China

关键词： gene expression; pea aphids; protease inhibitor protein; symbiotic bacteria

期刊名称：JOURNAL OF APPLIED ENTOMOLOGY （ 影响因子：2.0； 五年影响因子：2.1 ）

ISSN： 0931-2048

年卷期： 2025 年 149 卷 1 期

页码：

收录情况： SCI

摘要： Aphids are serious insect pests for agricultural and horticultural crops and may cause the major economic losses. Insecticides used to control aphids have caused environmental pollution and the insecticide residues in agricultural products. A new protease inhibitor gene from bacterium Xenorhabdus bovienii (Xbpi-1) and the protease inhibitor protein expressed by the gene against the pea aphid (Acyrthosiphon pisum Harris; Hemiptera: Aphididae) have been reported, however, effects of the PIP on the symbiotic bacteria, Buchnera aphidicola, and gene expression in pea aphids are unknown. By assessing the quantity of B. aphidicola, the primary symbiotic bacterium, in the aphid fed on an artificial diet containing 100 and 500 mu g/mL Xbpi-1, we observed a substantial reduction in its population by 27% and 46%, respectively, as analysed by real-time quantitative reverse transcription PCR (qRT-PCR). Furthermore, the growth of other aphid-associated bacteria was also significantly inhibited by Xbpi-1. To elucidate the mechanisms at the gene level, we conducted transcriptome analysis and identified differentially expressed genes (DEGs). Subsequent Gene Ontology (GO) analysis of the 213 DEGs shed light on the impact of Xbpi-1 on aphid metabolism processes and gene expression. Notably, the results highlighted several aphid nutrient metabolism pathways affected by Xbpi-1, which are relevant to vector-borne diseases. These pathways encompass crucial factors such as heat shock proteins, cuticle proteins and proteases. The results from this study revealed that the PIP had a novel mechanism against pea aphids by having adverse effects on the primary symbiotic bacteria in pea aphids and affecting aphid gene expression, showing that the PIP may be a promise bioinsecticide for aphid control in the future.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台