您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Overexpression of SoACLA-1 Gene Confers Drought Tolerance Improvement in Sugarcane

文献类型： 外文期刊

作者： Zhu, Kai 1 ; Huang, Chan 1 ; Phan, Thi-Thu 1 ; Yang, Li-Tao 1 ; Zhang, Bao-Qing 2 ; Xing, Yong-Xiu 1 ; Li, Yang-Rui 2 ;

作者机构： 1.Guangxi Univ, Coll Agr, Nanning 530004, Guangxi, Peoples R China

2.Chinese Acad Agr Sci, Guangxi Acad Agr Sci, Guangxi Key Lab Sugarcane Genet Improvement,Sugar, Minist Agr & Rural Affairs,Key Lab Sugarcane Biot, Nanning 530007, Peoples R China

关键词： SoACLA-1; Transgenic sugarcane; Water stress; Genetic transformation

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN： 0735-9640

年卷期：

页码：

收录情况： SCI

摘要： Drought is one of the most severe stresses which limit sugarcane production in China. ATP citrate lyase (ACL) is a major enzyme responsible for the production of acetyl-CoA in cytoplasm and plays an important role in plant metabolism and stress response. In this study, sugarcane ACL gene SoACLA-1 was cloned. The plant overexpression vector of SoACLA-1 was built and transformed into sugarcane calli by Agrobacterium-mediated transformation, and PCR analysis confirmed that SoACLA-1 gene had been stably present in the T0, T1, and T2 generations of the transgenic sugarcane. In order to evaluate the drought resistance of the transgenic lines and verify the function of SoACLA-1 gene in the transgenic sugarcane, T1 generation of the SoACLA-1 transgenic sugarcane lines was used as the material to investigate the physiological and biochemical characteristics at 0 day, 3 days, 6 days, and 9 days after water stress and rewatering for 3 days. Comprehensive evaluation of four indicators (chlorophyll, malondialdehyde, proline, soluble sugar) related to drought resistance was done with membership fuzzy function method. The results showed that the drought resistance of five transgenic sugarcane lines from strong to weak, in turn, was RT2 > RT4 > RT3 > RT1 > WT, and the recovery ability after drought, in turn, was RT1 > RT2 > RT4 > RT3 > WT. The T2 generation of the SoACLA-1 transgenic sugarcane lines was used to analyze the physiological and biochemical changes and the expression of drought-related genes under water stress. The results showed that the transgenic sugarcane lines were more tolerant to drought as compared with the wild-type plants. Our findings indicated that SoACLA-1 gene plays an important role as a positive factor in response to water stress, and overexpression of SoACLA-1 can enhance drought tolerance in transgenic sugarcane plants.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台