文献类型： 外文期刊

作者： Xie, Minmin ¹ ; Ding, Anming ¹ ; Guo, Yongfeng ¹ ; Sun, Jinhao ¹ ; Qiu, Wanya ¹ ; Chen, Mingli ¹ ; Li, Zhiyuan ¹ ; Li, Shanshan ¹ ; Zhou, Gongke ⁶ ; Xu, Yan ⁷ ; Wang, Meng ¹ ; Richel, Aurore ³ ; Gong, Daping ¹ ; Kong, Yingzhen ¹ ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Tobacco Res Inst, Biotechnol Ctr, Key Lab Tobacco Gene Resources, Qingdao 266101, Peoples R China

2.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China

3.Univ Liege, Lab Biomass & Green Technol, Gembloux Agrobio Tech, B-5030 Gembloux, Belgium

4.China Tobacco Jiangsu Ind Co Ltd, Technol Ctr, Nanjing 210019, Peoples R China

5.Yunnan Minzu Univ, Key Lab Nat Prod Synthet Biol Ethn Med Endophytes, State Ethn Affairs Commiss, Kunming 650031, Peoples R China

6.Qingdao Agr Univ, Coll Agron, Qingdao 266109, Peoples R China

7.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao Engn Res Ctr Biomass Resources & Environm, Key Lab Biofuels, Qingdao 266101, Peoples R China

期刊名称：PLANT CELL （ 影响因子：10.0； 五年影响因子：11.1 ）

ISSN： 1040-4651

年卷期： 2024 年

页码：

收录情况： SCI

摘要： The level of methylesterification alters the functional properties of pectin, which is believed to influence plant growth and development. However, the mechanisms that regulate demethylesterification remain largely unexplored. Pectin with a high degree of methylesterification is produced in the Golgi apparatus and then transferred to the primary cell wall where it is partially demethylesterified by pectin methylesterases (PMEs). Here, we show that in Arabidopsis (Arabidopsis thaliana) seed mucilage, pectin demethylesterification is negatively regulated by the transcription factor ZINC FINGER FAMILY PROTEIN5 (ZAT5). Plants carrying null mutations in ZAT5 had increased PME activity, decreased pectin methylesterification, and produced seeds with a thinner mucilage layer. We provide evidence that ZAT5 binds to a TGATCA motif and thereby negatively regulates methylesterification by reducing the expression of PME5, HIGHLY METHYL ESTERIFIED SEEDS (HMS)/PME6, PME12, and PME16. We also demonstrate that ZAT5 physically interacts with BEL1-LIKE HOMEODOMAIN2 (BLH2) and BLH4 transcription factors. BLH2 and BLH4 are known to modulate pectin demethylesterification by directly regulating PME58 expression. The ZAT5-BLH2/4 interaction provides a mechanism to control the degree of pectin methylesterification in seed coat mucilage by modifying each transcription factor's ability to regulate the expression of target genes encoding PMEs. Taken together, these findings reveal a transcriptional regulatory module comprising ZAT5, BLH2, and BLH4, that functions in modulating the demethylesterification of homogalacturonan in seed coat mucilage. Transcription factors ZINC FINGER FAMILY PROTEIN5 and BEL1-LIKE HOMEODOMAIN 2 and 4 modulate pectin methylesterase genes to control pectin demethylesterification in Arabidopsis seed coat mucilage.