文献类型： 外文期刊

作者： Zhang, Danhua ¹ ; Wang, Zhaoyilin ² ; Yamamoto, Naoki ¹ ; Wang, Mingyue ¹ ; Yi, Xiaoqun ¹ ; Li, Ping ² ; Lin, Runmao ³ ; Nasimi, Zohreh ¹ ; Okada, Kazunori ⁴ ; Mochida, Keiichi ⁵ ; Noutoshi, Yoshiteru ⁹ ; Zheng, Aiping ¹ ;

作者机构： 1.Sichuan Agr Univ, Coll Agron, Chengdu 611130, Peoples R China

2.Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Peoples R China

3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

4.Univ Tokyo, Agrobiotechnol Res Ctr, Bunkyo Ku, Tokyo 1138657, Japan

5.RIKEN Ctr Sustainable Resource Sci, Bioprod Informat Res Team, Yokohama, Kanagawa 2300045, Japan

6.RIKEN Cluster Sci Technol & Innovat Hub, Microalgae Prod Control Technol Lab, RIKEN Baton Zone Program, Yokohama, Kanagawa 2300045, Japan

7.Yokohama City Univ, Kihara Inst Biol Res, Yokohama, Kanagawa 2440813, Japan

8.Nagasaki Univ, Sch Informat & Data Sci, Nagasaki 8528521, Japan

9.Okayama Univ, Grad Sch Environm & Life Sci, Okayama 7008530, Japan

10.State Key Lab Crop Gene Explorat & Utilizat South, Chengdu 611130, Peoples R China

关键词： phytopathogenic fungi; pathogenicity; glycosyltransferases; plant innate immunity; virulence

期刊名称：PATHOGENS （ 影响因子：4.531； 五年影响因子：4.58 ）

ISSN：

年卷期： 2022 年 11 卷 9 期

页码：

收录情况： SCI

摘要： Anastomosis group AG-1 IA of Rhizoctonia solani Khun has a wide host range and threatens crop production. Various glycosyltransferases secreted by phytopathogenic fungi play an essential role in pathogenicity. Previously, we identified a glycosyltransferase RsIA_GT (AG11A_09161) as a secreted protein-encoding gene of R. solani AG-1 IA, whose expression levels increased during infection in rice. In this study, we further characterized the virulence function of RsIA_GT. It is conserved not only in Basidiomycota, including multiple anastomosis groups of R. solani, but also in other primary fungal taxonomic categories. RsIA_GT possesses a signal peptide (SP) for protein secretion, and its functionality was proven using yeast and Nicotiana benthamiana. The SP-truncated form of RsIA_GT (RsIA_GT(Delta S)) expressed in Escherichia coli-induced lesion-like phenotype in rice leaves when applied to punched leaves. However, Agrobacterium-mediated transient expressions of both the full-length RsIA_GT and RsIA_GT(Delta S) did not induce cell death in N. benthamiana leaves. Instead, only RsIA_GT(Delta S) suppressed the cell death induced by two reference cell death factors BAX and INF1 in N. benthamiana. RsIA_GT(Delta S)(R154A D168A D170A), a mutant RsIA_GT(Delta S) for the glycosyltransferase catalytic domain, still suppressed the BAX- or INF1-induced cell death, suggesting that the cell death suppression activity of RsIA_GT(Delta S) would be independent from its enzymatic activity. RsIA_GT(Delta S) also suppressed the H2O2 production and callose deposition and showed an effect on the induction of defense genes associated with the expression of BAX and INF1. The transient expression of RsIA_GT(Delta S) in N. benthamiana enhanced the lesion area caused by R. solani AG-1 IA. The secreted glycosyltransferase, RsIA_GT, of R. solani AG-1 IA is likely to have a dual role in virulence inside and outside of host cells.