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SAD2 functions in plant pathogen Pseudomonas syringae pv tomato DC3000 defense by regulating the nuclear accumulation of MYB30 in Arabidopsis thaliana

文献类型： 外文期刊

作者： Shi, Tiantian 1 ; Zheng, Yuan 2 ; Wang, Rui 1 ; Li, Sha 4 ; Xu, Andi 1 ; Chen, Luoying 1 ; Liu, Yuanhang 1 ; Luo, Rong 1 ; Huang, Chenchen 1 ; Sun, Yinglu 1 ; Zhao, Jinfeng 1 ; Guo, Xiaoying 5 ; Wang, Huan 4 ; Liu, Jun 1 ; Gao, Ying 1 ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improve, Beijing 100081, Peoples R China

2.Henan Univ, Sch Life Sci, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475001, Peoples R China

3.Henan Univ, Sanya Inst, Sanya 572025, Peoples R China

4.Chinese Acad Agr Sci CAAS, Biotechnol Res Inst, Beijing 100081, Peoples R China

5.Tianjin Agr Univ, Tianjin 300392, Peoples R China

6.Chengdu Natl Agr Sci & Technol Ctr, Chengdu 610213, Sichuan, Peoples R China

关键词： Nucleocytoplasmic transport; Nucleocytoplasmic transport receptors; Importin beta-like gene SAD2; MYB30; Plant pathogen defense

期刊名称：PLANT SCIENCE （ 影响因子：4.2； 五年影响因子：4.9 ）

ISSN： 0168-9452

年卷期： 2024 年 344 卷

页码：

收录情况： SCI

摘要： Accurate nucleocytoplasmic transport of signal molecules is essential for plant growth and development. Multiple studies have confirmed that nucleocytoplasmic transport and receptors are involved in regulating plant disease resistance responses, however, little is known about the regulatory mechanism in plants. In this study, we showed that the mutant of the importin beta-like protein SAD2 exhibited a more susceptible phenotype than wild-type Col-0 after treatment with Pseudomonas syringae pv tomato DC3000 (Pst DC3000). Coimmunoprecipitation (Co-IP) and bimolecular fluorescence complementation (BiFC) experiments demonstrated that SAD2 interacts with the hypersensitive response (HR)-positive transcriptional regulator MYB30. Subcellular localization showed that MYB30 was not fully localized in the nucleus in sad2-5 mutants, and western-blot experiments further indicated that SAD2 was required for MYB30 nuclear trafficking during the pathogen infection process. A phenotypic test of pathogen inoculation demonstrated that MYB30 partially rescued the disease symptoms of sad2-5 caused by Pst DC3000, and that MYB30 worked downstream of SAD2 in plant pathogen defense. These results suggested that SAD2 might be involved in plant pathogen defense by mediating MYB30 nuclear trafficking. Taken together, our results revealed the important function of SAD2 in plant pathogen defense and enriched understanding of the mechanism of nucleocytoplasmic transport-mediated plant pathogen defense.

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