A novel High-Affinity Anti-Capsaicinoids monoclonal antibody for High-Sensitivity Immunoassays: Experimental and computational validation
文献类型： 外文期刊
作者： Wen, Weihuan 1 ; Pan, Fei 4 ; Wang, Zhiruo 1 ; Li, Xinyu 2 ; Wang, Jiang 2 ; Talib, Hafiza Shaiza 2 ; Hu, Jie 2 ; Zhao, Yupeng 1 ; Liu, Zhiwei 1 ; Liang, Yifan 1 ; Wang, Yupu 1 ; Xiong, Wanqi 1 ; Luo, Lin 1 ; Xu, Zhenlin 1 ; Chen, Zhicheng 1 ;
作者机构： 1.South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Food Qual & Safety, Guangzhou 510642, Guangdong, Peoples R China
2.Shenzhen Bay Lab, Shenzhen 518132, Peoples R China
3.Hainan Univ, Minist Educ, Sch Pharmaceut Sci, Key Lab Trop Biol Resources, Haikou 570228, Peoples R China
4.Chinese Acad Agr Sci, Inst Apicultural Res, State Key Lab Resource Insects, Beijing, Peoples R China
5.Sun Yat Sen Univ, Sch Pharmaceut Sci Shenzhen, Shenzhen 518107, Peoples R China
关键词： Monoclonal antibody; Hybridoma; Capsaicinoids; Molecular docking; Molecular dynamics simulation; And immunoassay
期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）
ISSN： 0026-265X
年卷期： 2025 年 208 卷
页码：
收录情况： SCI
摘要： Capsaicinoids are the indicators of "gutter oil" contamination, and their sensitive detection is essential in edible oil quality control. We have developed HOPE1 monoclonal antibody (mAb), which binds major capsaicinoids with higher affinity than previously described YQQD8 mAb. Two immunoassays based on HOPE1 mAb were developed: an indirect competitive enzyme-linked immunosorbent assay with an IC50 of 121.03 pg/mL (linear range: 40.06-365.71 pg/mL) and a time-resolved fluorescence lateral flow immunoassay, which showed an IC50 of 0.57 ng/mL (linear range: 0.14-2.34 ng/mL). Both assays also yielded satisfactory recovery in spike tests. Further, molecular docking and molecular dynamic simulations of HOPE1 mAb highlighted the significance of hydrophobic interface in antibody-capsaicinoid recognition. Our study unveils a high-affinity mAb against capsaicinoids and its use in detecting gutter oil contamination. It is probably the first report on computational validation of capsaicinoids mAbs and their interaction with the target antigen.
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