Rapid detection of grapevine berry inner necrosis virus using a simple reverse transcription loop-mediated amplification method
文献类型： 外文期刊
作者： Zhang, Ying 1 ; Yuan, Qingyun 1 ; Ren, Fang 1 ; Hu, Guojun 1 ; Fan, Xudong 1 ; Dong, Yafeng 1 ;
作者机构： 1.Chinese Acad Agr Sci, Res Inst Pomol, Natl Ctr Eliminating Viruses Deciduous Fruit Tree, Xingcheng 125100, Liaoning, Peoples R China
关键词： Grapevine; Grapevine berry inner necrosis virus (GINV); Detection; RT-LAMP
期刊名称：PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY （ 影响因子：2.8； 五年影响因子：2.8 ）
ISSN： 0885-5765
年卷期： 2024 年 132 卷
页码：
收录情况： SCI
摘要： To develop a simple method for the detection of grapevine berry inner necrosis (GINV) that does not depend on expensive instruments, a reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assay was developed. A set of primers was used in RT-LAMP to specifically detect GINV and different GINV variants belonging to groups 1, 2 and 3. The sensitivity of the RT-LAMP was about 100 times greater than that of conventional RT-PCR. Furthermore, a rapid "pin-prick" assay without RNA extraction was developed to detect GINV in RT-LAMP. The assay performed well for the detection of GINV from different parts of field grapevine plants and micropropagated grapevine plantlets, and had a higher detection rate of GINV than RT-PCR in the tested grapevine samples.
- 相关文献
作者其他论文 更多>>
-
Simultaneous profiling of chromatin-associated RNA at targeted DNA loci and RNA-RNA Interactions through TaDRIM-seq
作者：Ding, Cheng;Chen, Guoting;Luan, Shiping;Gao, Runxin;Fan, Yudong;Zhang, Ying;Wang, Xiaoting;Li, Guoliang;Foda, Mohamed F.;Yan, Jiapei;Li, Xingwang;Chen, Guoting;Li, Guoliang;Chen, Guoting;Li, Guoliang;Foda, Mohamed F.;Li, Xingwang;Li, Xingwang
关键词：
-
Enhanced remediation of cadmium-contaminated farmland by smooth vetch (Vicia villosa var.) coupled with phosphorus/sulfur co-doped biochar: Synergetic performance and mechanism
作者：Bi, Fuxuan;Jiang, Zhao;Wang, Mengning;Lin, Qiutong;Liu, Xiaoyang;Qu, Jianhua;Zhang, Ying;Fu, Libo;Fan, Hongli;Cao, Weidong
关键词：P/S co-doped; Biochar; Smooth vetch; Cadmium; Synergistic remediation
-
An enhancer-promoter-transcription factor module orchestrates plant immune homeostasis by constraining camalexin biosynthesis
作者：Zhang, Ying;Tang, Meng;Cheng, Qinglin;Xie, Jiatao;Cheng, Jiasen;Li, Bo;Jiang, Daohong;Yu, Xiao;Zhang, Ying;Tang, Meng;Cheng, Qinglin;Xie, Jiatao;Cheng, Jiasen;Fu, Yanping;Li, Bo;Jiang, Daohong;Yu, Xiao;Zhang, Ying;Tang, Meng;Cheng, Qinglin;Chen, Wei;Xie, Jiatao;Li, Bo;Jiang, Daohong;Yu, Xiao;Chen, Wei;Chen, Wei;Zhang, Yi;Liu, Lijiang
关键词：plant innate immunity; enhancers; antifungal resistance; transcriptional regulation; cis-regulatory ele-ments; transcription factors
-
Integrated omics reveal the mechanisms underlying softening and aroma changes in pear during postharvest storage and the role of melatonin
作者：Xu, Jiayu;Zhang, Ying;Huo, Hongliang;Qi, Dan;Dong, Xingguang;Tian, Luming;Liu, Chao;Cao, Yufen
关键词：Zaoshu Shanli; Whole transcriptome; Firmness; Transmission electron microscopy; Ethylene; Metabolite; CeRNA
-
Application of flavonoid compounds suppresses the cotton aphid, Aphis gossypii
作者：Zhang, Ying;Yang, Guoqing;Zhang, Ying;Wang, Xinhang;Bian, Zhipeng;Se, Chenchen;Lu, Yanhui;Lu, Yanhui
关键词：
Aphis gossypii ; flavonoids; antifeedant activity; population Growth; fecundity; ecofriendly pest control -
Identification and Evaluation of Flesh Texture of Crisp Pear Fruit Based on Penetration Test Using Texture Analyzer
作者：Mou, Yulu;Dong, Xingguang;Zhang, Ying;Tian, Luming;Huo, Hongliang;Qi, Dan;Xu, Jiayu;Liu, Chao;Yin, Chen;Yang, Xiang;Li, Niman
关键词：pear; germplasm resources; flesh texture; sensory evaluation; puncture method
-
Synergistic Insecticidal Activity Against Hyphantria cunea by Cry9Aa3 Mutants and Cry1Ah Combinations
作者：Xu, Pengdan;Han, Jiaxing;Liao, Min;Zhang, Jie;Xu, Pengdan;Wang, Zeyu;Zhang, Ying;Han, Jiaxing;Shu, Changlong;Zhang, Jie;Geng, Lili
关键词：
Hyphantria cunea ;Bacillus thuringiensis ;cry9 -type genes; mutant; synergistic effect
