文献类型： 外文期刊

作者： Huang, Yu-Feng ¹ ; He, Fan ¹ ; Cui, Hao ¹ ; Zhang, Yan-Yu ¹ ; Yang, Hua-Yi ¹ ; Liang, Zong-Suo ⁴ ; Dai, Wei ⁵ ; Cheng, Chun-Song ¹ ; Xie, Ying ¹ ; Liu, Liang ¹ ; Liu, Zhong-Qiu ⁷ ; Zhou, Hua ¹ ;

作者机构： 1.Macau Univ Sci & Technol, Fac Chinese Med, Taipa 999078, Macao, Peoples R China

2.Macau Univ Sci & Technol, State Key Lab Qual Res Chinese Med, Taipa 999078, Macao, Peoples R China

3.Shanghai Univ Tradit Chinese Med, Inst Int Standardizat Tradit Chinese Med, Shanghai 201203, Peoples R China

4.Zhejiang Sci Tech Univ, Coll Life Sci, Hangzhou 310018, Zhejiang, Peoples R China

5.Mianyang Acad Agr Sci, Inst Tradit Chinese Med, Mianyang 621023, Sichuan, Peoples R China

6.Macau Univ Sci & Technol, Joint Lab Translat Canc Res Chinese Med, Minist Educ Peoples Republ China, Taipa 999078, Macao, Peoples R China

7.Guangzhou Univ Chinese Med, Joint Lab Translat Canc Res Chinese Med, Minist Educ Peoples Republ China, Guangzhou 510006, Guangdong, Peoples R China

关键词： Aconitum carmichaelii lateral root; Yunaconitine; Crassicauline A; 8-deacetylyunaconitine; 8-deacetylcrassicauline A; LD50

期刊名称：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS （ 影响因子：3.571； 五年影响因子：3.552 ）

ISSN： 0731-7085

年卷期： 2022 年 208 卷

页码：

收录情况： SCI

摘要： Yunaconitine (YAC), crassicauline A (CCA), 8-deacetylyunaconitine (DYA), and 8-deacetylcrassicauline A (DCA), as hidden toxic Aconitum alkaloids, are detected in some products of processed Aconitum carmichaelii lateral root and poisoning cases. The distribution and toxicity of these four components in Aconitum herbs should be further systematically studied for medication safety. This study developed a new UHPLC-QQQ-MS/MS method to determine ten Aconitum alkaloids, including aconitine, mesaconitine, hypaconitine, benzoylaconine, benzoylmesaconine, benzoylhypaconine, YAC, CCA, DYA, and DCA, for Aconitum herbs simultaneously. YAC and CCA were founded in some samples of unprocessed A. carmichaelii lateral root (7.04%), A. carmichaelii root (9.43%), A. brachypodum root (6.00%), and A. ouvrardianum root (100%). Four hidden toxic Aconitum alkaloids were detected in processed A. carmichaelii lateral root (2.56%) and A. vilmorinianum root (100%). Four hidden toxic Aconitum alkaloids played significant roles in the classification of Aconitum herbs by OPLS-DA analysis. The acute toxicity test was performed by up-and-down procedure (UDP). The oral administration of the half lethal dose (LD50) of YAC, CCA, DYA, and DCA to female ICR mice was 2.37 mg/kg, 5.60 mg/kg, 60.0 mg/kg, and 753 mg/kg, respectively. The LD50, by intravenous injection was 0.200 mg/kg, 0.980 mg/kg, 7.60 mg/kg, and 34.0 mg/kg, respectively. The LD50 of unprocessed A. carmichaelii lateral root, A. vilmorinianum root, and A. brachypodum root to mice orally was 1.89 g/kg, 0.950 g/ kg, and 0.380 g/kg, respectively. Symptoms of Aconitum alkaloid poisoning in mice were decreased activity, fur erect, palpebral edema, vomiting, polypnea, and convulsions. The main change of organs was flatulence. No poisoning or death occurred in mice at the maximum dosage (27.0 g/kg) of A. ouvrardianum root orally. To better control the quality and safety of Aconitum herbs, this study provides favorable support for improving the existing standards to strengthen the supervision of the four hidden toxic Aconitum alkaloids. (C) 2021 Elsevier B.V. All rights reserved.