The Construction of a Mutant Library of Linseed and the Molecular Mechanism of High Oleic Acid Mutants from a Multi-Omics Perspective
文献类型： 外文期刊
作者： Liu, Caiyue 1 ; Yang, Xinsen 1 ; Tang, Qiaoling 1 ; Cao, Xiuxia 2 ; Qian, Aiping 2 ; Yang, Zhiwei 2 ; Wang, Limin 3 ; Zhang, Jianping 3 ; Pei, Xinwu 1 ; Gan, Lu 1 ;
作者机构： 1.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China
2.Ningxia Acad Agr & Forestry Sci, Guyuan Branch, Guyuan 756000, Peoples R China
3.Gansu Acad Agr Sci, Crop Inst, Lanzhou 730070, Peoples R China
关键词： high-oleic acid; flax; EMS; multi-omics
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2025 年 14 卷 16 期
页码：
收录情况： SCI
摘要： Flax (Linum usitatissimum L.) is a globally important oilseed crop, valued for its edible and industrial uses. Flax seeds are rich in unsaturated fatty acids. In this study, ethyl methyl sulfone was employed to construct a mutant library from the flax cultivar Longya 10 (WT). Screening efforts identified M45, a stable mutant with an oleic acid content of 43.22% at 40 days after flowering, representing a 21.23% increase over the wild-type. RNA-Seq analysis revealed the presence of two homologs of the SAD (stearoyl-ACP desaturase) family and two homologs of the FAD2 (fatty acid desaturase 2) family, which showed differential expression in a trend consistent with the phenotype of M45. A BSA-Seq analysis was conducted to identify genes with SNPs (single nucleotide polymorphisms) and Indel (insertions/deletions) variant loci that were associated with increased oleic acid. The combination of BSA-Seq, RNA-Seq, and metabolomic analyses identified L.us.o.g.scaffold122.86, a gene that may be co-expressed with L.us.o.g.scaffold7.26 to affect oleic acid accumulation via FAD2.
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