Plasmonic colorimetry and G-quadruplex fluorescence-based aptasensor: A dual-mode, protein-free and label-free detection for OTA
文献类型： 外文期刊
作者： Li, Ming 1 ; Qiu, Youxin 1 ; Liu, Guoxing 1 ; Xiao, Yu 1 ; Tian, Ye 1 ; Fang, Song 2 ;
作者机构： 1.Jiangsu Univ, Inst Environm Hlth & Ecol Secur, Sch Emergency Management, Sch Environm & Safety Engn, Zhenjiang 212013, Peoples R China
2.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Lab Tobacco Qual & Safety Risk Assessment, Tobacco Res Inst, Qingdao 266101, Peoples R China
3.Suzhou Univ Sci & Technol, Jiangsu Collaborat Innovat Ctr Technol & Mat Water, Sch Environm Sci & Engn, Suzhou 215009, Peoples R China
关键词： Ochratoxin A; Surface plasmon resonance; G -quadruplex; Gold nanoparticle; Dual -mode detection
期刊名称：FOOD CHEMISTRY （ 影响因子：8.8； 五年影响因子：8.6 ）
ISSN： 0308-8146
年卷期： 2024 年 448 卷
页码：
收录情况： SCI
摘要： G-quadruplexes (G4) have received significant attention in the field of aptasensors owing to their unique physicochemical characteristics. A dual-mode, protein-free and label-free aptamer sensor based on plasmonic colorimetry and G4 fluorescence (PC@GF-aptasensor) was proposed for ochratoxin A (OTA). Colorimetry mode was achieved through the surface plasmon resonance (SPR) effect, which related to the OTA-Apt-based G4-OTA. The fluorescence mode was reflected by the insertion of thioflavin T (ThT) into G4-OTA. The OTA could be interpreted via three readouts: (1) naked eye (LOD of 2.0 ng mL-1), (2) smartphone (LOD of 1.65 ng mL-1), and (3) spectrofluorometer (LOD of 0.93 ng mL-1). The PC@GF-aptasensor exhibited several advantages, such as a standardised recognition group, simplified operation, low background signal, and practicality. The proposed PC@GF-aptasensor integrated SPR-based multicolour interpretation and ThT-inserted fluorescence reflection to obtain a dual-mode optical biosensor, which may provide valuable insights for the development of other targets with G4-based aptamers.
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