Expression and sex pheromone-binding characteristics of pheromone-binding protein 3 in Tuta absoluta (Lepidoptera: Gelechiidae)
文献类型： 外文期刊
作者： Ou, Xiaolan 1 ; Li, Xianya 2 ; Xu, Bo 1 ; Wang, Yusheng 1 ; Zhang, Guifen 1 ; Liu, Wanxue 1 ; Wan, Fanghao 1 ; Jiang, Hongbo 2 ; Haddi, Khalid 3 ; Huang, Cong 1 ; Zhang, Yibo 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
2.Southwest Univ, Coll Plant Protect, Key Lab Entomol & Pest Control Engn, Chongqing 400716, Peoples R China
3.Lavras Fed Univ UFLA, Dept Entomol, BR-37200900 Lavras, MG, Brazil
关键词： Tuta absoluta; Pheromone-binding protein; Fluorescence competitive binding assays; Molecular docking
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）
ISSN： 0048-3575
年卷期： 2025 年 210 卷
页码：
收录情况： SCI
摘要： The olfactory system plays a crucial role in insect survival and reproduction. Odorant-binding proteins (OBPs) are essential for odor discrimination and hold the potential to be targets for pest management. Tuta absoluta (Lepidoptera: Gelechiidae), a devastating invasive pest of Solanaceae crops, has limited research on its OBPs. In this study, 34 OBP genes were identified in T. absoluta, including TabsGOBP1, TabsGOBP2, TabsPBP1a, TabsPBP1b, TabsPBP1c, and TabsPBP3, which belong to the Lepidoptera-specific GOBP/PBP subclass. Expression profiling revealed TabsPBP3 to be predominantly expressed in male antennae and the female pheromone gland-ovipositor complex, with peak expression at 6:00 AM associated with courtship and mating behavior. Fluorescence competitive binding assays demonstrated that TabsPBP3 strongly binds to the main pheromone component (3E, 8Z, 11Z)-tetradecatrien-1-yl acetate (TDTA) but exhibits weak or no affinity for other components. Molecular docking identified key active sites in TabsPBP3, including Phe37, Tyr61, Ile77, Leu84, Ile86, Leu87, Phe101, Ala136, Ile139, and Ala140, which facilitate interaction with TDTA. These findings establish TabsPBP3 as a key player in TDTA detection and provide foundational data for innovative pest control strategies targeting T. absoluta.
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