文献类型： 外文期刊

作者： Tang, Ling ¹ ; Li, Ji ¹ ; Tang, Ning ¹ ; Li, Zhengguo ¹ ;

作者机构： 1.Chongqing Acad Agr Sci, Maize Res Inst, Chongqing 401329, Peoples R China; Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Nanjing 210095, Peoples R China; Chongqing Univ, Sch life Sci, Chongqing 400000, Peoples R China; Chongqing Univ Arts & Sci, Res Inst Special Plants, Collaborat Innovat Ctr Special Plant Ind Chongqing, Chongqing Key Lab Econ Plant Biotechnol, Chongqing 402160, Peoples R China

关键词： Carrots Daucus carota L.; protoplast; transient expression; subcellular localization; BiFC

期刊名称：INDIAN JOURNAL OF BIOTECHNOLOGY （ 影响因子：0.324； 五年影响因子：0.503 ）

ISSN： 0972-5849

年卷期： 2021 年 20 卷 4 期

页码：

收录情况： SCI

摘要： Carrots are the top ten most economically important vegetable crops worldwide by market value and production area. Protoplasts are generally isolated from Tobacco Bright Yellow 2 (BY2) cell lines, tobacco leaf cells and onion bulb cells. However, the complicated and expensive preparation method limits these protoplasts transient expression (TE). This study shows that carrot protoplasts have many advantages for TE, including easy access, low preparation cost, high repeatability, uniform size and strong vitality. We developed an efficient method for producing carrot protoplasts. Meanwhile, carrot protoplasts were successfully utilized for protein subcellular localization analysis and bimolecular fluorescence complementation (BiFC).