文献类型： 外文期刊
作者： Shi, Chuanlin 1 ; Zou, Wenli 1 ; Liu, Xiangpei 1 ; Zhang, Hong 1 ; Li, Xiaofang 1 ; Fu, Guiling 1 ; Fei, Qili 1 ; Qian, Qian 1 ; Shang, Lianguang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Genome Anal Lab, Shenzhen Branch,Guangdong Lab Lingnan Modern Agr,M, Shenzhen, Peoples R China
2.Zhengzhou Univ, State Key Lab Cotton Biol, Zhengzhou Res Base, Zhengzhou, Peoples R China
3.Shanxi Agr Univ, Coll Agr, Taiyuan, Shanxi, Peoples R China
4.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou, Zhejiang, Peoples R China
5.Yazhouwan Natl Lab, Sanya, Hainan, Peoples R China
关键词： N-6-methyladenonsine; m(6)A editing; CRISPR/dCas13a; plant growth; Arabidopsis
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）
ISSN： 1467-7644
年卷期： 2024 年
页码：
收录情况： SCI
摘要： N-6-methyladenonsine (m(6)A) is the most prevalent internal modification of messenger RNA (mRNA) and plays critical roles in mRNA processing and metabolism. However, perturbation of individual m(6)A modification to reveal its function and the phenotypic effects is still lacking in plants. Here, we describe the construction and characterization of programmable m(6)A editing tools by fusing the m(6)A writers, the core catalytic domain of the MTA and MTB complex, and the AlkB homologue 5 (ALKBH5) eraser, to catalytically dead Cas13a (dCas13a) to edit individual m(6)A sites on mRNAs. We demonstrated that our m(6)A editors could efficiently and specifically deposit and remove m(6)A modifications on specific RNA transcripts in both Nicotiana benthamiana and Arabidopsis thaliana. Moreover, we found that targeting SHORT-ROOT (SHR) transcripts with a methylation editor could significantly increase its m(6)A levels with limited off-target effects and promote its degradation. This leads to a boost in plant growth with enlarged leaves and roots, increased plant height, plant biomass, and total grain weight in Arabidopsis. Collectively, these findings suggest that our programmable m(6)A editing tools can be applied to study the functions of individual m(6)A modifications in plants, and may also have potential applications for future crop improvement.
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