Differential gene expression in leaves and roots of winter rape in response to phosphorus starvation
文献类型： 外文期刊
作者： Qin, L. 1 ; Zhang, Ch. L. 1 ; Zhang, B. 3 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Oil Crops Res, Crop Physiol & Sustainable Agr Div, Wuhan 430062, Peoples R China
2.Shandong Acad Agr Sci, Crop Res Inst, Jinan 250100, Peoples R China
3.Shandong Acad Agr Sci, High Tech Res Ctr, Jinan 250100, Peoples R China
关键词： Brassica napus; DDRT-PCR; phosphorus starvation; semi-quantitative RT-PCR; gene expression
期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）
ISSN： 1021-4437
年卷期： 2011 年 58 卷 1 期
页码：
收录情况： SCI
摘要： Genes induced by phosphorus deficiency in leaves and roots of winter rape (Brassica napus) seedlings were isolated and analyzed. The mRNA differential display technique was used to visualize cDNA fragments derived from the phosphorus-tolerant cv. Zy 821 and the phosphorus-sensitive cv. No. 1182 grown under conditions of phosphorous starvation. Approximately 2000 cDNA bands were visualized by differential display using 78 primer pair combinations. A total of 61 phosphorus-starvation-induced cDNA fragments differentially expressed were isolated. Among these, 40 were derived from roots and only 21 from leaves. Sequence analysis of 16 fragments revealed that they represented distinct cDNAs. A subsample of five cDNAs was analyzed by semi-quantitative RT-PCR, which showed that they were truly different products. Transcripts coding for enzymes involved in photosynthesis (thylakoid membrane phosphoprotein) and root hair initiation (xyloglucan endotransglycosylase), and two regulators (RNA-binding/nucleic acid-binding mRNA and a structural constituent of ribosomal mRNA) were found to be differentially expressed. These results show that the mechanism of cv. Zy 821 adaptation to P starvation is complex. Although its P-tolerance trait is controlled by a major gene, many other genes influencing P acquisition and remobilization, carbon and secondary metabolism, developmental processes, and regulatory pathways are also involved.
- 相关文献
作者其他论文 更多>>
-
Aridity modulates grassland biomass responses to combined drought and nutrient addition
作者：Bondaruk, V. F.;Yahdjian, L.;Onatibia, G. R.;Biancari, L.;Bondaruk, V. F.;Yahdjian, L.;Onatibia, G. R.;Biancari, L.;Bondaruk, V. F.;Jentsch, A.;Wanke, S.;Xu, C.;Ke, Y. G.;Wu, H. H.;Xu, C.;Wu, H. H.;Wilfahrt, P.;Borer, E. T.;Seabloom, E. W.;Isbell, F.;Churchill, A. C.;Yu, Q.;Han, L. B.;Yu, Q.;Smith, M. D.;Ohlert, T.;Wilkins, K.;Alberti, J.;Daleo, P.;Dieguez, H.;Carbognani, M.;Forte, T.;Petraglia, A.;Kuebert, A.;Werner, C.;Kuebert, A.;Power, S. A.;Chandregowda, M. H.;Tissue, D.;Eisenhauer, N.;Auge, H.;Eisenhauer, N.;Auge, H.;Churchill, A. C.;Greenville, A. C.;Wardle, G. M.;Greenville, A. C.;Verhoeven, E.;Wardle, G. M.;Koerner, S. E.;Young, A. L.;Peri, P.;Salesa, D.;Tedder, M.;Maziko, Y.;Valdecantos, A.;Wheeler, G. R.;Wheeler, G. R.;An, H.;Diao, H. J.;Gutknecht, J.;Ke, Y. G.;Liu, J. L.;Tian, D. S.;Wei, C. Z.;Wei, C. Z.;Wilkins, K.;Zhang, F. W.;Zhang, B.;Zhu, J. T.;Zong, N.;Zuo, X. A.;Hautier, Y.
关键词：
-
Effects of coated sodium butyrate on growth performance, intestinal morphology, and caecal short-chain fatty acids of growing Pekin ducks
作者：Ji, Z. Q.;Hao, Y. S.;Wang, Y. T.;Zhang, B.;Tang, J.;Huang, W.;Zhang, Q.;Hou, S. S.;Xie, M.;Wang, Y. T.;Xie, M.
关键词：Ducks; coated sodium butyrate; growth performance; intestinal morphology; short-chain fatty acids
-
EVALUATING THE RESOURCES OF RAPHANUS SATIVUS L. FOR DUAL PURPOSE OF SIGHTSEEING AND GREEN MANURE BASED ON GREY CORRELATION ANALYSIS UNDER THE RURAL REVITALIZATION STRATEGY IN GUIZHOU
作者：Zhang, Q.;Yao, D-J;Kuang, S-J;Liao, H.;Zhang, A-H;Wang, W-H;Zhu, Q.;Ran, B.;Qin, L.
关键词：ecological value; flowering period; area of flower; returnable nutrient characteristics
-
Effects of riboflavin deficiency on the lipid metabolism of duck breeders and duck embryos
作者：Zhang, B.;Tang, J.;Wu, Y. B.;Cao, J. T.;Xing, G. N.;Sun, P. X.;Huang, W.;Xie, M.;Hou, S. S.;Cao, J. T.
关键词：duck breeder; duck embryo; lipid metabolism; protein expression; riboflavin
-
Nutrient composition of Chinese oak silkworm, Antheraea pernyi, a traditional edible insect in China: a review
作者：Li, Q.;Li, Y-P;Liu, Y-Q;Qin, L.;Ambuhl, D.;Ambuhl, D.;Li, M-W
关键词：Antheraea pernyi; amino acids; fatty acids; minerals; vitamins; hormones
-
Research Note: Effects of riboflavin on reproductive performance and antioxidant status of duck breeders
作者：Zhang, B.;Wu, Y. B.;Sun, P. X.;Wei, J.;Huang, W.;Xie, M.;Tang, J.;Hou, S. S.;Zhao, R.;Fouad, A. M.
关键词：duck breeder; riboflavin; reproductive performance; antioxidant status
-
Pantothenic acid requirement of male White Pekin ducks from hatch to 21 days of age
作者：Tang, J.;Zhang, B.;Wu, Y. B.;Feng, Y. L.;Huang, W.;Zhou, Z. K.;Xie, M.;Hou, S. S.;Xue, M.;Shi, W. B.
关键词：Duck; Pantothenic acid; Requirement; Growth performance
