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Identification and genomic analysis of a novel temperate bacteriophage infecting Labrenzia aggregata isolated from the Mariana Trench

文献类型： 外文期刊

作者： Gu, Bingyu 1 ; Wang, Haowen 1 ; Lv, Jiayi 3 ; Zheng, Yanfen 1 ; Zhang, Xiao-Hua 1 ; Zhan, Yuanchao 1 ;

作者机构： 1.Ocean Univ China, Coll Marine Life Sci, Qingdao, Peoples R China

2.Ocean Univ China, Fisheries Coll, Lab Pathol & Immunol Aquat Anim, Qingdao, Peoples R China

3.Ocean Univ China, Haide Coll, Qingdao, Peoples R China

4.Chinese Acad Agr Sci, Marine Agr Res Ctr, Tobacco Res Inst, Qingdao, Peoples R China

关键词： bacteriophage induction; deep-sea; genomic analysis; Labrenzia aggregata; Labrenmarinevirus

期刊名称：FRONTIERS IN MARINE SCIENCE （ 影响因子：3.7； 五年影响因子：4.7 ）

ISSN：

年卷期： 2024 年 11 卷

页码：

收录情况： SCI

摘要： In marine environments, viruses play a pivotal role, yet deep-sea bacteriophages remains largely uncharacterized. The bacterium Labrenzia aggregata RF14, isolated from the Mariana Trench at a depth of 4,000 meters, harbors prophage regions based on a previous study. In this study, we induced a temperate bacteriophage from it using mitomycin C. The bacteriophage exhibited an icosahedral structure with a non-extendable tail and was named vB_LagS-V1. The genome size of it is 39,329 bps with a 59.46% G+C content, encoding 60 putative open reading frames. Genomic and phylogenetic analyses demonstrated that vB_LagS-V1 along with many bacteriophages infecting Hyphomicrobiales, constituted a newly unclassified family, which we designated as Hyphoviridae. Within this novel family, vB_LagS-V1 is distinct with isolated phages and clustered with two uncultured prophages within Labrenzia, forming an unclassified new genus, given a name of Labrenmarinevirus. The codon usage correlation and absence of tRNAs found in vB_LagS-V1, also prevail in some deep-sea bacteriophages, highlighting their adaptations to the deep-sea prokaryotic hosts. Moreover, vB_LagS-V1 encoded two auxiliary metabolic genes, cysteine dioxygenase and phosphoadenosine phosphosulfate reductase, which might help the phage and its host adapt to high hydrostatic pressure in the deep-sea environments. Our study will significantly contribute to the understanding of deep-sea bacteriophages and their interactions with hosts in extreme environments.

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