Integrated Data Reveal the Flavonoid Biosynthesis Metabolic Pathway in Semen Ziziphi Spinosae Using Transcriptomic and Metabolomic
文献类型: 外文期刊
作者: Tian, Wei 1 ; Liu, Lingdi 1 ; Song, Zhongxing 2 ; Tang, Zhishu 2 ; Wen, Chunxiu 1 ; Jiang, Tao 1 ;
作者机构: 1.Hebei Acad Agr & Forestry Sci, Inst Cash Crops, Shijiazhuang 050051, Hebei, Peoples R China
2.Shaanxi Univ Chinese Med, State Key Lab Res & Dev Characterist Qin Med Reso, Xianyang 712046, Peoples R China
关键词: Spinosin; secondary metabolites; transcriptomic; metabolomic; gene expression
期刊名称:PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY ( 影响因子:1.7; 五年影响因子:1.4 )
ISSN: 0031-9457
年卷期: 2023 年 92 卷 2 期
页码:
收录情况: SCI
摘要: The extract from Semen Ziziphi Spinosae (SZS) contains many secondary metabolites (flavonoids, alkaloids, and terpenoids), widely reported for their high medicinal value. In order to know the number and type of flavonoids and the regulating mechanism of flavonoid biosynthesis during the growth and development of SZS, we identified and analyzed the flavonoid metabolites and genes in SZS using metabolomic and transcriptomic. A total of 13,232 differentially expressed genes and 83 flavonoid metabolites were identified at three different growth and development stages (T1, T2, and T3) of SZS. The main flavonoid metabolic components of SZS were cate-chin, L-epicatechin, (-)-epigallocatechin, (+)-gallocatechin, spinosin and its derivatives. A total of 53 unigenes encoding 15 enzymes were identified as candidate genes involved in flavonoid biosynthesis in SZS. Among, PAL, C4H, 4CL, CHS, CHI, FLS, FNS, ANS, ANR, LAR, and UFGT genes were all downregulated in T3 than in T1. The expression levels of these genes influence the accumulation of flavonoids in SZS. Our results should pro-vide valuable information for flavonoid metabolites and candidate genes involved in flavonoid biosynthesis on SZS
- 相关文献
作者其他论文 更多>>
-
Metabolomics and transcriptomics provide insights into the flavonoid biosynthesis pathway in the roots of developing Aster tataricus
作者:Jia, Kaixuan;Zhang, Xiaoling;Liu, Shuqi;Liu, Xiaoqing;Yang, Taixin;Ge, Shujun;Jia, Kaixuan;Zhang, Xiaoling;Meng, Yijiang;Liu, Shuqi;Liu, Xiaoqing;Yang, Taixin;Ge, Shujun;Meng, Yijiang;Wen, Chunxiu;Liu, Lingdi
关键词:Aster tataricus; Differentially expressed genes; Flavonoids; Metabolite; Transcriptome
-
American ginseng with different processing methods ameliorate immunosuppression induced by cyclophosphamide in mice via the MAPK signaling pathways
作者:Zhang, Yan-Ting;Lu, Yu-Shun;Li, Zhi-Man;Ren, Duo-Duo;Zhang, Yue;Sha, Ji-Yue;Huo, Xiao-Hui;Sun, Yin-Shi;Tian, Wei;Li, Shan-Shan
关键词:American ginseng; steam-processing; CTX; immunosuppression; MAPK
-
Integrating metabolomics, 16S rRNA sequencing, network pharmacology, and metorigin to explore the mechanism of Cinnamomi Cortex in treating chronic atrophic gastritis rats
作者:Liu, Yuetao;Zhang, Hui;Lu, Wentian;Liu, Yuetao;Zhang, Hui;Lu, Wentian;Jiang, Tao
关键词:Cinnamomi cortex; Chronic atrophic gastritis; Network pharmacology; Metabolomics; Metorigin; 16S rRNA sequencing
-
Genetic mapping, transcriptomic sequencing and metabolic profiling indicated a glutathione S-transferase is responsible for the red-spot-petals in Gossypium arboreum
作者:Zhang, Sujun;Cai, Xiao;Wang, Haitao;Liu, Cunjing;Tang, Liyuan;Li, Xinghe;Zhang, Xiangyun;Zhang, Jianhong;Jiang, Tao;Chen, Jie
关键词:
-
Metabolomics Analysis of Metabolites in Forsythia suspense Fruit Using UPLC/ESI-Q TRAP-MS/MS
作者:Liu, Lingdi;Wen, Chunxiu;Tian, Wei;Xie, Xiaoliang;Wen, Saiqun;Jiang, Tao
关键词:Forsythiae Fructus; traditional Chinese medicine; UPLC/ESI-Q TRAP-MS/MS; component; metabolite
-
Metabolome analysis of genus Forsythia related constituents in Forsythia suspensa leaves and fruits using UPLC-ESI-QQQ-MS/MS technique
作者:Liu, Lingdi;Wen, Chunxiu;Jiang, Tao;Tian, Wei;Xie, Xiaoliang;Lu, Ruike;Feng, Jiaxing;Jin, Aihong;Wen, Saiqun;Liu, Lingdi;Wen, Chunxiu;Jiang, Tao;Tian, Wei;Xie, Xiaoliang;Lu, Ruike;Feng, Jiaxing;Jin, Aihong;Wen, Saiqun;Sun, Yu;Wei, Wei;Cui, Xusheng
关键词:
-
Integrated transcriptomic and metabolomic data reveal the flavonoid biosynthesis metabolic pathway in Perilla frutescens (L.) leaves
作者:Jiang, Tao;Liu, Lingdi;Tian, Wei;Xie, Xiaoliang;Wen, Saiqun;Wen, Chunxiu;Guo, Kunyuan
关键词: