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Promotion of apoplastic oxidative burst by artificially selected GhCBSX3A enhances Verticillium dahliae resistance in upland cotton

文献类型： 外文期刊

作者： Zhang, Yihao 1 ; Yuan, Yuan 1 ; Xi, Hongfang 2 ; Zhang, Yaning 1 ; Gao, Chenxu 1 ; Ma, Meng 1 ; Huang, Qian 2 ; Li, Fuguang 1 ; Yang, Zhaoen 1 ;

作者机构： 1.Zhengzhou Univ, Natl Key Lab Cotton Biobreeding & Integrated Utili, Zhengzhou Res Base, Zhengzhou 450001, Peoples R China

2.Chinese Acad Agr Sci, Inst Cotton Res, Natl Key Lab Cotton Biobreeding & Integrated Utili, Anyang 455000, Peoples R China

3.Chinese Acad Agr Sci, Western Agr Res Ctr, Changji 831100, Xinjiang, Peoples R China

4.Natl Wheat Innovat Ctr, Coll Agron, State Key Lab Wheat & Maize Crop Sci, Zhengzhou 450001, Henan, Peoples R China

5.Ctr Crop Genome Engn, Zhengzhou 450001, Henan, Peoples R China

关键词： upland cotton; Verticillium dahliae; reactive oxygen species; artificial selection

期刊名称：PLANT JOURNAL （ 影响因子：7.2； 五年影响因子：7.9 ）

ISSN： 0960-7412

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Verticillium wilt (VW) is a devasting disease affecting various plants, including upland cotton, a crucial fiber crop. Despite its impact, the genetic basis underlying cotton's susceptibility or defense against VW remains unclear. Here, we conducted a genome-wide association study on VW phenotyping in upland cotton and identified a locus on A13 that is significantly associated with VW resistance. We then identified a cystathionine beta-synthase domain gene at A13 locus, GhCBSX3A, which was induced by Verticillium dahliae. Functional analysis, including expression silencing in cotton and overexpression in Arabidopsis thaliana, confirmed that GhCBSX3A is a causal gene at the A13 locus, enhancing SAR-RBOHs-mediated apoplastic oxidative burst. We found allelic variation on the TATA-box of GhCBSX3A promoter attenuated its expression in upland cotton, thereby weakening VW resistance. Interestingly, we discovered that altered artificial selection of GhCBSX3A_R (an elite allele for VW) under different VW pressures during domestication and other improved processes allows specific human needs to be met. Our findings underscore the importance of GhCBSX3A in response to VW, and we propose a model for defense-associated genes being selected depending on the pathogen's pressure. The identified locus and gene serve as promising targets for VW resistance enhancement in cotton through genetic engineering.

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