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Interaction of R2R3-MYB transcription factor EgMYB111 with ABA receptors enhances cold tolerance in oil palm

文献类型： 外文期刊

作者： Zhou, Lixia 1 ; Li, Rui 1 ; Yang, Xuanwen 1 ; Peng, Yanling 3 ; Wang, Yiwen 3 ; Xu, Qi 3 ; Yang, Yaodong 1 ; Iqbal, Amjad 2 ; Su, Xiangnian 3 ; Zhou, Yongfeng 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Natl Key Lab Trop Crop Breeding, Haikou 571101, Peoples R China

2.Chinese Acad Trop Agr Sci, Coconut Res Inst, Hainan Key Lab Trop Oil Crops Biol, Wenchang 571339, Peoples R China

3.Chinese Acad Agr Sci, Minist Agr & Rural Affairs, Agr Genom Inst Shenzhen, Natl Key Lab Trop Crop Breeding,Shenzhen Branch,Gu, Shenzhen 518120, Peoples R China

4.Univ Melbourne, Sch Math & Stat, Melbourne Integrat Genom, Melbourne, Vic, Australia

5.Abdul Wali Khan Univ Mardan, Dept Food Sci & Technol, Mardan, Pakistan

关键词： Elaeis guineensis; Oil palm; MYB transcription factors; EgMYB111; ABA signaling pathway; Cold tolerance

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 305 卷

页码：

收录情况： SCI

摘要： The oil palm is a prominent tropical oil crop with holds considerable economic value. MYB transcription factors are key regulators in growth and plant stress adaptation mechanisms in plants. However, the roles and operational mechanisms of MYB genes in oil palm are not yet well understood. In this study, EgMYB111 was cloned from oil palm, and its behavior under cold stress was examined in genetically engineered tobacco and oil palm embryoids. Physiological and biochemical analysis demonstrated that genetically engineered lines exhibited substantially greater cold tolerance than control plants. EgMYB111 was noticed to localize within the nucleus, and cold stress significantly enhanced the expression of the GUS gene managed by the EgMYB111 expression regulator. Interestingly, EgMYB111 was involved in the reaction to stress via an abscisic acid (ABA)-mediated pathway. Yeast two-hybrid experiments confirmed the involvement of EgMYB111 and the ABA receptor proteins PYR1 and PYL9. Moreover, the transient transformation of oil palm protoplasts combined with qRT-PCR analysis revealed that the over-activity of EgMYB111 induced a significant induction of the genes EgSnRK2.1, EgSnRK2.3, and EgSnRK2.5. In addition, dual-luciferase analyses, yeast one-hybrid assays, and electrophoretic mobility shift assays (EMSA) established that EgMYB111 binds to the promoters of EgSnRK2.1, EgSnRK2.3, and EgSnRK2.5, thereby regulating their transcription and enhancing low-temperature resilience in oil palm. The work concludes that the EgMYB111 performs a key role in augmenting cold adaptability in oil palm by governing the transcription of key genes utilizing an ABA-regulated pathway.

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