Unveiling side-chain architectures of corn husk hemicellulose to drive enzyme cocktail design for degradation of biomass
文献类型： 外文期刊
作者： Guo, Yingjie 1 ; Zhang, Zheng 1 ; Li, Xuezhi 1 ; Zhao, Jian 1 ;
作者机构： 1.Shandong Univ, State Key Lab Microbial Technol, Qingdao 266237, Peoples R China
2.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao 266101, Peoples R China
关键词： Corn husk hemicellulose; Structural characterization; One-step enzymatic hydrolysis
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2025 年 310 卷
页码：
收录情况： SCI
摘要： By analyzing residual hemicellulose after enzymatic hydrolysis of corn husks, we identified previously unreported side-chain architectures in glucuronoarabinoxylan and xyloglucan of corn husk hemicellulose, including alpha-galactosyl-mannose and alpha-xylosyl-arabinose linkages, which shield glycosidic bonds from enzymatic cleavage. These discoveries unveil novel structural complexes in hemicellulose and broaden the understanding of hemicellulose recalcitrance beyond existing literature. Further, we designed a tailored enzyme cocktail by heterologously expressing three targeted hemicellulases (GH51 alpha-L-arabinofuranosidase, GH27 alpha-D-galactosidase, and GH31 alpha-D-xylosidase) based on hemicellulose structural information, and synergistic integration of these enzymes with Penicillium oxalicum cellulase via response surface optimization. This enzyme system achieved unprecedented yields of 81.82 % glucose and 54.89 % arabinose through one-step hydrolysis without chemical pretreatment, surpassing previous benchmarks by over 30 %. This study advances the understanding of hemicellulose's intricate structural complexity and enhances insights into multi-enzyme synergies during biomass degradation, offering a pivotal framework for developing pretreatment-free, eco-friendly enzymatic hydrolysis processes and enabling high-value utilization of corn husks.
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