Efficient differentiation between Salmonella Pullorum and Salmonella Gallinarum by a fimH-based PCR-HRM
文献类型： 外文期刊
作者： Zhang, Di 1 ; Zhuang, Linlin 2 ; Jiang, Yi 1 ; Yang, Yi 3 ; Xu, Ming 4 ; Dou, Xinhong 4 ; Gong, Jiansen 1 ;
作者机构： 1.Chinese Acad Agr Sci, Poultry Inst, 58 Cangjie Rd, Yangzhou 225125, Peoples R China
2.Jiangsu Vocat Coll Agr & Forestry, Sch Anim Husb & Vet Med, Jurong, Peoples R China
3.Yangzhou Univ, Coll Vet Med, Yangzhou, Peoples R China
4.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou, Peoples R China
关键词：
期刊名称：AVIAN PATHOLOGY （ 影响因子：2.2； 五年影响因子：2.9 ）
ISSN： 0307-9457
年卷期： 2025 年 54 卷 4 期
页码：
收录情况： SCI
摘要： Salmonella Pullorum (S. Pullorum) and Salmonella Gallinarum (S. Gallinarum) are the biovars of Salmonella enterica serovar Gallinarum that are responsible for pullorum disease and fowl typhoid, respectively, in poultry. Traditional serological methods fail to quickly differentiate between these biovars due to their identical O antigenic factors (O9 and O12). Although single nucleotide polymorphism (SNP)-based methods have been used to distinguish between the biovars, they often lack the required accuracy and effectiveness. In this study, we developed a PCR high resolution melt (PCR-HRM) assay, which targeted a SNP at position 665 of the fimH gene, for rapid differentiation between S. Pullorum and S. Gallinarum. Our method showed 100% specificity and was able to detect as little as 0.033 pg of S. Pullorum DNA and 0.027 pg of S. Gallinarum DNA. The PCR-HRM results for 547 clinical isolates were in complete agreement with traditional serological methods. This PCR-HRM assay significantly reduced identification time and provided high throughput, efficient testing. This makes it a practical and reliable tool for accurate differentiation between S. Pullorum and S. Gallinarum in clinical settings.
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