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Transcriptomic Analysis Reveals Candidate Genes Responding Maize Gray Leaf Spot Caused by Cercospora zeina

文献类型： 外文期刊

作者： He, Wenzhu 1 ; Zhu, Yonghui 1 ; Leng, Yifeng 3 ; Yang, Lin 1 ; Zhang, Biao 1 ; Yang, Junpin 1 ; Zhang, Xiao 2 ; Lan, Hai; 1 ;

作者机构： 1.Sichuan Acad Agr Sci, Crop Res Inst, Chengdu 610066, Peoples R China

2.Sichuan Agr Univ, Maize Res Inst, Chengdu 611130, Peoples R China

3.Xichang Univ, Coll Agr Sci, Xichang 615000, Peoples R China

关键词： maize; RNA-Seq; gray leaf spot; WGCNA; disease resistance

期刊名称：PLANTS-BASEL （ 影响因子：3.935； ）

ISSN：

年卷期： 2021 年 10 卷 11 期

页码：

收录情况： SCI

摘要： Gray leaf spot (GLS), caused by the fungal pathogen Cercospora zeina (C. zeina), is one of the most destructive soil-borne diseases in maize (Zea mays L.), and severely reduces maize production in Southwest China. However, the mechanism of resistance to GLS is not clear and few resistant alleles have been identified. Two maize inbred lines, which were shown to be resistant (R6) and susceptible (S8) to GLS, were injected by C. zeina spore suspensions. Transcriptome analysis was carried out with leaf tissue at 0, 6, 24, 144, and 240 h after inoculation. Compared with 0 h of inoculation, a total of 667 and 419 stable common differentially expressed genes (DEGs) were found in the resistant and susceptible lines across the four timepoints, respectively. The DEGs were usually enriched in 'response to stimulus' and 'response to stress' in GO term analysis, and 'plant-pathogen interaction', 'MAPK signaling pathways', and 'plant hormone signal transduction' pathways, which were related to maize's response to GLS, were enriched in KEGG analysis. Weighted-Genes Co-expression Network Analysis (WGCNA) identified two modules, while twenty hub genes identified from these indicated that plant hormone signaling, calcium signaling pathways, and transcription factors played a central role in GLS sensing and response. Combing DEGs and QTL mapping, five genes were identified as the consensus genes for the resistance of GLS. Two genes, were both putative Leucine-rich repeat protein kinase family proteins, specifically expressed in R6. In summary, our results can provide resources for gene mining and exploring the mechanism of resistance to GLS in maize.

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