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Comparative microRNAs profile of Schistosoma japonicum male worms derived from single-sex and bisexual infections: Implications of the multifunctional role of microRNA

文献类型： 外文期刊

作者： Zhong, Haoran 1 ; Zhu, Danlin 1 ; Dong, Bowen 1 ; Wu, Luobin 1 ; Lu, Ke 1 ; Fu, Zhiqiang 1 ; Liu, Jinming 1 ; Guan, Guiquan 3 ; Jin, Yamei 1 ;

作者机构： 1.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Natl Reference Lab Anim Schistosomiasis, Key Lab Anim Parasitol,Minist Agr & Rural Affairs, Shanghai, Peoples R China

2.Shanghai Normal Univ, Coll Life Sci, Shanghai, Peoples R China

3.Lanzhou Univ, Chinese Acad Agr Sci, State Key Lab Anim Dis Control & Prevent, Coll Vet Med,Lanzhou Vet Res Inst, Lanzhou, Gansu, Peoples R China

4.Gansu Prov Res Ctr Basic Disciplines Pathogen Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou, Gansu, Peoples R China

关键词： Schistosoma japonicum; MiRNA; Single-sex male worms; Mated male worms

期刊名称：PARASITOLOGY RESEARCH （ 影响因子：2.0； 五年影响因子：2.1 ）

ISSN： 0932-0113

年卷期： 2025 年 124 卷 4 期

页码：

收录情况： SCI

摘要： Schistosoma japonicum is a dioecious parasite that requires constant pairing between male and female worms for female maturation and egg production. MicroRNAs (miRNAs) play crucial roles in regulating various biological processes, including parasite development and host-pathogen interactions, but their functions in schistosomes remain largely unexplored. This study aimed to investigate the differentially expressed miRNAs (DEMs) between mated male (MM) and single-sex male (SM) worms to gain insights into their regulatory roles in schistosome reproduction. Total RNA was extracted from 28-day-old MM and SM worms, followed by small RNA sequencing to identify DEMs. Bioinformatics analyses were used to predict the biological functions of DEM target genes. Comparative analysis with previously published miRNA datasets helped identify potentially significant miRNAs. Quantitative PCR (qPCR) validated the expression of selected miRNAs and mRNA levels of some target genes. A total of 20 DEMs were identified, with 9 upregulated in MM worms and 11 in SM worms. These DEMs may regulate processes such as intracellular transport, RNA processing, and cellular homeostasis. The study provides novel insights into the biological differences between SM and MM worms, suggesting that these miRNAs could be key regulators of parasite development and host adaptation, with potential diagnostic and therapeutic applications in schistosomiasis.

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