文献类型： 外文期刊
作者： Wu, Lina 1 ; Wang, Xinjie 3 ; Wu, Xiangchuan 1 ; Xu, Shiqi 1 ; Liu, Ming 4 ; Cao, Xizhong 4 ; Tang, Taishan 4 ; Huang, Xingxu 1 ; Huang, He 1 ;
作者机构： 1.Nanjing Normal Univ, Sch Food Sci & Pharmaceut Engn, Nanjing 210023, Peoples R China
2.Zhejiang Lab, Hangzhou 311100, Peoples R China
3.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Genome Anal Lab,Minist Agr & Rural Affairs,Guangdo, Shenzhen 518120, Peoples R China
4.Guangzhou Med Univ, Affiliated Hosp 1, Guangzhou Inst Resp Hlth, State Key Lab Resp Dis,Natl Clin Res Ctr Resp Dis, Guangzhou 510120, Peoples R China
关键词： MnO2 nanorods; CRISPR-Cas12a; SARS-CoV-2; Nanozyme; Detection
期刊名称：ACS APPLIED MATERIALS & INTERFACES （ 影响因子：10.383； 五年影响因子：10.382 ）
ISSN： 1944-8244
年卷期：
页码：
收录情况： SCI
摘要： The CRISPR-Cas system was developed into a molecular diagnostic tool with high sensitivity, low cost, and high specificity in recent years. Colorimetric assays based on nanozymes offer an attractive point-of-care testing method for their low cost of use and user-friendly operation. Here, a MnO2 nanozyme-mediated CRISPR-Cas12a system was instituted to detect SARS-CoV2. MnO2 nanorods linked to magnetic beads via a single-stranded DNA (ssDNA) linker used as an oxidase-like nanozyme inducing the color change of 3,3',5,5'-tetramethylbenzidine, which can be distinguished by the naked eye. The detection buffer color will change when the Cas12a is activated by SARS-CoV-2 and indiscriminately cleave the linker ssDNA. The detection limit was 10 copies per microliter and showed no cross-reaction with other coronaviruses. The nanozyme-mediated CRISPR-Cas12a system shows high selectivity and facile operation, with great potential for molecular diagnosis in point-of-care testing applications.
- 相关文献
作者其他论文 更多>>
-
NMR spectroscopy combined with chemometrics for quality assessment of common vegetable oils: A review
作者：Shi, Ting;Wang, Xinjie;Dai, Tenghui;Ma, Xiaodong;Zhang, Tao
关键词：Vegetable oil; Nutritional quality parameters; Oxidation evaluation; Authenticity; NMR; Chemometrics
-
Divergent effects of biomass-derived carbon dots application and sweetpotato planting on accumulations of soil microbial necromass carbon in Vertisol
作者：Wang, Jing;Zhu, Meng;Zhu, Xiaoya;Zhang, Qiangqiang;Yu, Yongchao;Zhao, Peng;Liu, Ming;Jin, Rong;Tang, Zhonghou
关键词：Soil microbial necromass; Carbon dots; Sweetpotato; Soil organic carbon; Microbial community structure
-
Thermal tolerance of turbot based on cardiac performance and analysis of molecular regulatory mechanisms
作者：Jiang, Yuhang;Huang, Zhihui;Ma, Aijun;Cao, Junwen;Zhang, Hao;Zhao, Yinuo;Hu, Yan;Jiang, Yuhang;Cao, Junwen;Zhao, Yinuo;Jiang, Yuhang;Huang, Zhihui;Ma, Aijun;Cao, Junwen;Zhang, Hao;Zhao, Yinuo;Hu, Yan;Liu, Ming
关键词：
Scophthalmus maximus (L.); Thermal stress; beta-AR; Heart rate; Cardiac performance -
Achieving synchronous nitrogen and phosphorus removal by aerobic enrichment of electrotrophic/heterotrophic bacteria and denitrifying polyphosphate-accumulating organisms in repeatedly oxygen-rich microbial fuel cells
作者：Jiang, Xiaomei;Xiong, Xia;Liu, Ming;Yang, Nuan;Gao, Yi;Yao, Ling;Luo, Di;Lei, Yunhui;Jiang, Xiaomei;Xiong, Xia;Liu, Ming;Yang, Nuan;Gao, Yi;Yao, Ling;Lei, Yunhui;Jiang, Xiaomei
关键词：Household domestic wastewater; Repeatedly oxygen-rich condition; Mixotrophic ammonium oxidation; Electrotrophic and heterotrophic denitrification; Phosphorus removal
-
Development of a rapid on-site detection method for largemouth bass virus based on RPA-CRISPR/Cas12a system
作者：Li, Na;Dai, Chunmei;Mao, Yuelin;Zhang, Yimeng;Yan, Huiyuan;Wang, Huijiao;Qin, Yinghui;Yao, Lunguang;Huang, Baicheng;Wang, Xinjie
关键词：largemouth bass virus;
MCP gene; recombinase polymerase amplification; CRISPR/Cas12a; detection -
Single-molecule assay guided crRNA optimization enhances specific microRNA detection by CRISPR-Cas12a
作者：Chen, Kaizhao;Zhong, Mingtian;Xie, Jiaqi;Huo, Yongkun;Ma, Xiaodong;Chen, Kaizhao;Zhong, Mingtian;Xie, Jiaqi;Huo, Yongkun;Ma, Xiaodong;Sun, Wenjun;Lu, Xiuqi;Chen, Ziting;Sun, Bo;Huang, Xingxu;Huang, Xingxu;Wang, Xinjie;Liu, Ming;Ma, Peixiang
关键词：MicroRNA; CRISPR; Cas12a; Stem-loop; Detection
-
Palm Multidiagnostic of Mycoplasma pneumoniae, Chlamydia pneumoniae, Haemophilus influenzae, and Streptococcus pneumoniae Using One-Tube CRISPR/Cas12a
作者：Kan, Xingchi;Wu, Yue;Zeng, Zihan;Huang, Xingxu;Huang, Baicheng;Zhang, Xiyue;Lou, Chen;Wu, Lina;Wang, Xinjie;Wang, Xinjie
关键词：
