文献类型: 中文期刊
作者: 黄淑贤 1 ; 刘荣 1 ; 李冠 1 ; 疏琴 1 ; 徐斐 1 ; 宗绪晓 1 ; 杨涛 1 ;
作者机构: 1.中国农业科学院作物科学研究所
关键词: 豌豆;CRISPR/Cas9;发状根;大片段敲除
期刊名称: 作物学报
ISSN: 0496-3490
年卷期: 2024 年 007 期
页码: 1658-1668
收录情况: 北大核心 ; CSCD
摘要: 豌豆是世界上主要食用豆类作物之一,因其丰富的营养价值和良好的生态价值而受到越来越多的重视.CRISPR/Cas9作为一种生物育种的新型技术手段,已广泛应用于各种作物的品种改良中,但在豌豆中的应用报道非常少见.本研究以"中豌6号"品种为试验材料,采用CRISPR/Cas9技术手段成功地实现了豌豆基因组中Psat01G0240600-T1、Psat03G0303300-T1和Psat03G0304700-T1基因的大片段缺失,其大片段敲除效率分别是24.1%、13.0%和3.6%.进一步对结果分析发现,不同靶点的编辑效率相差较大,并且大片段缺失的效率取决于2个靶点中较低编辑效率的靶点.本研究利用发状根体系探索了CRISPR/Cas9在豌豆中的应用,首次在豌豆中实现了大片段敲除,对于豌豆的研究具有重要意义.
- 相关文献
[1]CRISPR/Cas9介导的大豆基因组定点编辑研究. 蔡宇鹏. 2016
[2]发状根在药用植物基因工程研究进展. 李天航,杨晶,王艳芳,郭咏昕,李昌禹,李海燕,李校堃. 2010
[3]大豆多基因编辑表达载体的构建及应用. 陈向前,姜奇彦,孙现军,牛风娟,张慧媛,胡正,张辉. 2022
[4]发状根在药用植物基因工程研究进展. 李天航,杨晶,王艳芳,郭咏昕,李昌禹,李海燕,李校堃. 2010
[5]Creation of targeted inversion mutations in plants using an RNA-guided endonuclease. Zhang, Congsheng,Liu, Changlin,Weng, Jianfeng,Liu, Fang,Li, Xinhai,Xie, Chuanxiao,Zhang, Congsheng,Cheng, Beijiu. 2017
[6]Deletion of the Bombyx mori odorant receptor co-receptor (BmOrco) impairs olfactory sensitivity in silkworms. Hao, Dejun,Liu, Qun,Hao, Dejun,Liu, Qun,Zeng, Baosheng,Huang, Yongping,Wang, Guirong.
[7]Production of alpha 1,3-galactosyltransferase and cytidine monophosphate-N-acetylneuraminic acid hydroxylase gene double-deficient pigs by CRISPR/Cas9 and handmade cloning. Gao, Hanchao,Zhao, Chengjiang,Zhao, Yanli,Li, Zesong,Cai, Zhiming,Mou, Lisha,Gao, Hanchao,Zhao, Chengjiang,Xiang, Xi,Li, Yong,Pan, Dengke,Dai, Yifan,Hara, Hidetaka,Cooper, David K. C..
[8]Targeted mutagenesis by CRISPR/Cas9 system in the model legume Medicago truncatula. Meng, Yingying,Hou, Yaling,Wang, Hui,Niu, Lifang,Lin, Hao,Ji, Ronghuan,Liu, Bin,Wen, Jiangqi.
[9]Live attenuated pseudorabies virus developed using the CRISPR/Cas9 system. Tang, Yan-Dong,Liu, Ji-Ting,Wang, Tong-Yun,An, Tong-Qing,Sun, Ming-Xia,Wang, Shu-Jie,Fang, Qiong-Qiong,Hou, Lin-lin,Tian, Zhi-Jun,Cai, Xue-Hui,Liu, Ji-Ting.
[10]Recent insights into cotton functional genomics: progress and future perspectives. Javaria Ashraf,Song, Guoli,Dongyun Zuo,Qiaolian Wang,Waqas Malik,Youping Zhang,Muhammad Ali Abid,Hailiang Cheng,Qiuhong Yang,Guoli Song. 2018
[11]Genome Editing in Cotton with the CRISPR/Cas9 System. Wei Gao,Lu Long,Song, Chunpeng,Xinquan Tian,Ji Liu,Prashant K. Singh,Jose R. Botella,Chunpeng Song. 2017
[12]Recombinant Pseudorabies Virus (PRV) Expressing Firefly Luciferase Effectively Screened for CRISPR/Cas9 Single Guide RNAs and Antiviral Compounds. Tang, Yan-Dong,Liu, Ji-Ting,Fang, Qiong-Qiong,Wang, Tong-Yun,Sun, Ming-Xia,An, Tong-Qing,Tian, Zhi-Jun,Cai, Xue-Hui,Liu, Ji-Ting. 2016
[13]Generation of buffalo mammary epithelial cells with targeted knockout of 18s rDNA by a CRISPR/Cas9 adenovirus system. Zhu, Peng,Duan, An-Qin,Deng, Ting-Xian,Lu, Xing-Rong,Ma, Xiao-Ya,Liang, Sha-Sha,Pang, Chun-Ying,Liang, Xian-Wei,Zhu, Peng. 2017
[14]CRISPR/Cas9: A powerful tool for crop genome editing. Song, Gaoyuan,Jia, Meiling,Chen, Kai,Kong, Xingchen,Khattak, Bushra,Xie, Chuanxiao,Li, Aili,Mao, Long. 2016
[15]Reconstitution of UCP1 using CRISPR/Cas9 in the white adipose tissue of pigs decreases fat deposition and improves thermogenic capacity. Zheng, Qiantao,Huang, Jiaojiao,Zhang, Hongyong,Zhang, Rui,Cao, Chunwei,Qin, Guosong,Yao, Jing,Song, Ruigao,Jia, Qitao,Wang, Xiao,Li, Yongshun,Zhang, Nan,Wang, Hongmei,Zhou, Qi,Zhao, Jianguo,Zheng, Qiantao,Huang, Jiaojiao,Zhang, Hongyong,Zhang, Rui,Cao, Chunwei,Qin, Guosong,Yao, Jing,Song, Ruigao,Jia, Qitao,Wang, Xiao,Wang, Hongmei,Zhou, Qi,Jin, Wanzhu,Zhao, Jianguo,Lin, Jun,Song, Ruigao,Ye, Rongcai,Jin, Wanzhu,Lin, Jun,Ye, Rongcai,Zhang, Xueying,Speakman, John R.,Hambly, Catherine,Speakman, John R.,Piao, Zhengyu,Wang, Yanfang.
[16]CRISPR/Cas9-mediated targeted mutagenesis of GmFT2a delays flowering time in soya bean. Cai, Yupeng,Chen, Li,Liu, Xiujie,Guo, Chen,Hou, Wensheng,Cai, Yupeng,Chen, Li,Liu, Xiujie,Guo, Chen,Sun, Shi,Wu, Cunxiang,Jiang, Bingjun,Han, Tianfu,Hou, Wensheng. 2018
[17]A simple and efficient method for CRISPR/Cas9-induced mutant screening. Hua, Yufeng,Wang, Chun,Wang, Kejian,Huang, Jian. 2017
[18]Generation of High-Amylose Rice through CRISPR/Cas9-Mediated Targeted Mutagenesis of Starch Branching Enzymes. Sun, Yongwei,Liu, Zupei,Zhang, Xin,Li, Jingying,Guo, Xiuping,Du, Wenming,Du, Jinlu,Xia, Lanqin,Jiao, Guiai,Francis, Frederic,Zhao, Yunde. 2017
[19]CRISPR/Cas9-mediated multiple single guide RNAs potently abrogate pseudorabies virus replication. Tang, Yan-Dong,Liu, Ji-Ting,Wang, Tong-Yun,Sun, Ming-Xia,Tian, Zhi-Jun,Cai, Xue-Hui. 2017
[20]Highly Efficient CRISPR/Cas9-Mediated Homologous Recombination Promotes the Rapid Generation of Bacterial Artificial Chromosomes of Pseudorabies Virus. Guo, Jin-Chao,Tang, Yan-Dong,Zhao, Kuan,Wang, Tong-Yun,Liu, Ji-Ting,Gao, Jia-Cong,Chang, Xiao-Bo,Cui, Hong-Yu,Tian, Zhi-Jun,Can, Xue-Hui,An, Tong-Qing. 2016
作者其他论文 更多>>
-
印度南瓜GAox家族基因鉴定及其在不同蔓长种质中的表达
作者:孙廷珍;疏琴;马玮;史玉滋;张蒙;向成钢;薄凯亮;段颖;王长林
关键词:印度南瓜;赤霉素氧化酶;基因家族;基因进化;主蔓长度;表达分析
-
蚕豆锈病致病机制及抗性策略研究进展
作者:张志林;席亚东;项超;刘荣;李祥;宗绪晓;杨涛
关键词:蚕豆锈病;抗性机制;遗传育种;病害综合防治
-
冷季补饲精料对放牧牦牛血清生化指标及瘤胃菌群的影响
作者:张犇;丁自强;康彦东;赵索南;杨涛;白玛多杰;索昂求培;郭宪
关键词:牦牛;补饲;血清生化指标;瘤胃菌群
-
西藏山南市优质油菜引种研究
作者:杨涛;胡琼;拥嘎;梅德胜;杨艳斌;达娃桑珠;朱霞
关键词:油菜;品种;产量;品质;西藏
-
中棉所164简介
作者:夏成明;范术丽;杨涛;詹有俊;孙建船;张璇;马启峰;张金忠;乔凯凯
关键词:棉花;中棉所164;品种选育;特征特性;产量;纤维品质;栽培技术
-
枯草期玉树牦牛血液生化指标及瘤胃菌群构成分析
作者:丁自强;张犇;郭韶珂;胡丽妍;裴杰;熊琳;索昂求培;杨涛;赵索南;郭宪
关键词:玉树牦牛;血液生化指标;瘤胃微生物
-
酒棉26号的选育与栽培技术要点
作者:张璇;杨涛;詹有俊;孙建船;范术丽;张金忠;杨军;李渊
关键词:棉花;酒棉26号;品种选育;特征特性;产量;纤维品质;栽培技术




