文献类型: 中文期刊
作者: 王艳玲 1 ; 孟志刚 1 ; 李妍妍 1 ; 孟钊红 1 ; 王远 1 ; 孙国清 1 ; 朱涛 1 ; 梁成真 1 ; 蔡永萍 1 ; 郭三堆 1 ; 张锐 1 ; 林毅 1 ;
作者机构: 1.安徽农业大学生命科学学院;中国农业科学院生物技术研究所
关键词: CRISPR/Cas9;精氨酸酶;GhARG;侧根发育;棉花
期刊名称: 中国科学:生命科学
ISSN: 1674-7232
年卷期: 2017 年 11 期
页码: 1200-1203
收录情况: 北大核心 ; CSCD
摘要: 基因敲除是植物功能基因组研究和农艺性状定向遗传改良的关键步骤,近年来,CRISPR/Cas9介导的目标基因敲除在拟南芥(Arabidopsis thaliana)、水稻(Oryza sativa)等植物中取得飞速的发展,然而在棉花(Gossypium spp.)中目前尚没有成功的报道.陆地棉(G.hirsutum L.)是典型的异源四倍体模式作物,包括A亚基因组和D亚基因组.利用CRISPR/Cas9技术同时成功地敲除A和D亚基因组精氨酸酶编码基因(GhARG),Gh_A05G2143和Gh_D05G2397.无论高氮还是低氮条件下,T1代双基因纯和株系侧根生长发育显著地提高.因此,本研究的CRISPR/Cas9系统可以同时高效地编辑棉花中多个同源基因,从而实现关键农艺性状简单快速得改良.
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