Drought and Heat Stress-Mediated Modulation of Alternative Splicing in the Genes Involved in Biosynthesis of Metabolites Related to Tea Quality
文献类型： 外文期刊
第一作者： Ding, Y. Q.
作者： Ding, Y. Q.;Fan, K.;Wang, Y.;Sun, L. T.;Qiu, C.;Ding, Z. T.;Fang, W. P.;Zhu, X. J.;Chen, L.
作者机构：
关键词： tea; Camellia sinensis (L.); O. Kuntze; alternative splicing; amino acids; flavonoids; volatile compounds
期刊名称：MOLECULAR BIOLOGY （ 影响因子：1.374； 五年影响因子：1.266 ）
ISSN： 0026-8933
年卷期： 2022 年 56 卷 2 期
页码：
收录情况： SCI
摘要： Alternative splicing (AS) regulates mRNAs at the post-transcriptional level to affect both their amounts and the protein function. However, little is known about the roles of AS in regulation of biosynthesis of amino acids, flavonoids, and volatile compounds in tea plants. In this study, we used Iso-seq and transcriptome deep sequencing (RNA-seq) to identify AS events, and analyzed the expression of respective mRNAs in tea plants under drought (DS), heat stress (HS), and their combination (HD). By RT-PCR, we validated the AS events in nine genes involved in the biosynthesis of amino acids and flavonoids. The genes accumulating AS transcripts under DS, HS, and HD conditions included those encoding for anthocyanidin reductase (ANR), dihydrofavonol-4-reductase-like (DFRA), and chalcone isomerase (CHI). Similarly, genes directly or indirectly involved in the biosynthesis of volatile compounds such as lipoxygenase (LOX), terpenoid/terpene synthase (TPS), and 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXR) also had AS events. Our study revealed that AS might specifically regulate the biosynthesis of amino acids in tea plants under stressful conditions. Moreover, we suggest that the AS events within the ANR and DFRA transcripts might play an important role in the regulation of flavonoid biosynthesis under DS, HS, and HD conditions. This study improved our understanding of the genetic drivers of the changes in the content of bioactive ingredients of tea plants subjected to abiotic stresses.
分类号：
- 相关文献
作者其他论文 更多>>
-
Cloning and Functional Identification of Yunwu Tribute Tea CsPPO Gene
作者：Wang, Y.;Li, L.;Wan, C.;Wang, Y.;Li, Y.;Wang, Y.;Li, Y.;Zhao, D.
关键词：Camellia sinensis var; niaowangensis; cold stress; polyphenoloxidase gene; functional identification
-
Characterization and High Expression of a Novel Alkalinegh11 Xylanase in Pichia pastoris
作者：Wang, Y.;Deng, Y. -D.;Zhang, W. H.;Gong, Z. -H.;Qian, C.;Peng, R. -H.;Yao, Q. -H.;Tian, Y. -S.;Han, H. -J.;Zhang, W. -Y.;Wang, Y.;Han, H. -J.
关键词：alkali-tolerant; codon optimization; thermostability; xylanases
-
The Effect of a-Fe2O3 Nanoparticles on the Physiological Characteristics and Active Ingredients of Muskmelon on the Cadmium-Polluted Soil
作者：Zou, Z.;Zhou, Y.;Hao, Y.;Li, J.;Qi, M.;Qiu, L.;Cheng, Y.;Wang, Y.;Dai, Z.;Wang, Y.;Dai, Z.
关键词：Cucumis melo L.; soil cadmium pollution; muskmelon; & alpha;-Fe2O3 nanoparticles; growth parameters; antioxidant system; active ingredients
-
Genome-wide association study identifies variants associated with semen volume in white-feathered broilers
作者：Zhang, G. M.;Liu, P. H.;Zheng, J. M.;Zhao, G. P.;Xing, W. H.;Wen, J.;Li, Q. H.;Chen, L.
关键词：GWAS; semen volume; white-feathered broiler
-
Inulin-induced differences on serum extracellular vesicles derived miRNAs in dairy cows suffering from subclinical mastitis
作者：Yu, W.;Nan, X.;Wang, Y.;Xiong, B.;Yu, W.;Schroyen, M.
关键词：Gene ontology; Inflammation; Kyoto Encyclopedia of Genes and Genomes; Pathway; RNA sequencing
-
Inulin-induced differences on serum extracellular vesicles derived miRNAs in dairy cows suffering from subclinical mastitis
作者：Yu, W.;Nan, X.;Wang, Y.;Xiong, B.;Yu, W.;Schroyen, M.
关键词：Gene ontology; Inflammation; Pathway; RNA sequencing; Kyoto Encyclopedia of Genes and Genomes
-
Improvement of feed intake, digestibility, plasma metabolites, and lactation performance of dairy cows fed mixed silage of sugar beet pulp and rice straw inoculated with lactic acid bacteria
作者：Wang, Y.;Liu, J.;Liu, X. L.;Guo, J. H.;Wang, Y.;Li, Y. J.;Zhang, Y. G.;Xia, K.;Wang, X. N.;Lin, X.;Zhao, W. J.
关键词：sugar beet pulp; mixture silage; inoculation; milk yield; milk protein
