Functional analysis of the nonstructural protein NSs of tomato zonate spot virus
文献类型: 外文期刊
第一作者: Li, Jing
作者: Li, Jing;Chen, Si;Chen, Si;Qiu, Run-Shuang;Zhang, Li-Zhen;Chen, Yue;Zheng, Xue;Li, Ting-Ting;Zhao, Li-Hua;Zhang, Zhong-Kai
作者机构:
期刊名称:PLOS ONE ( 影响因子:3.752; 五年影响因子:4.069 )
ISSN: 1932-6203
年卷期: 2022 年 17 卷 1 期
页码:
收录情况: SCI
摘要: Tomato zonate spot virus (TZSV), a member of the genus orthotospovirus, causes severe damage to vegetables and ornamental crops in southwest China. The NSs protein is an RNA silencing suppressor in various orthotospovirus like TZSV, but its mechanism and role in virus infection are poorly understood. Here, we observed that an NSs-GFP fusion protein was transiently expressed on the plasma membrane and Golgi bodies in Nicotiana benthamiana plants. The TZSV NSs gene was silenced and infiltrated into N. benthamiana and N. tabacum cv. K326. RT-qPCR and Indirect enzyme-linked immunosorbent assay (ID-ELISA) showed that the transcription and the protein expression of the NSs gene were inhibited by more than 90.00%, and the symptoms on silenced plants were alleviated. We also found that the expression of the Zingipain-2-like gene significantly decreased when the NSs gene was silenced, resulting in co-localization of the NSs-GFP and the Zingipain-2-like-mCherry fusion protein. The findings of this study provide new insights into the mechanism of silencing suppression by NSs, as well as its effect on systemic virus infection, and also support the theory of disease resistance breeding and control and prevention of TZSV in the field.
分类号:
- 相关文献
作者其他论文 更多>>
-
Folate Biofortification in Soybean: Challenges and Prospects
作者:Agyenim-Boateng, Kwadwo Gyapong;Zhang, Shengrui;Shaibu, Abdulwahab S. S.;Li, Jing;Li, Bin;Sun, Junming;Shohag, Md. Jahidul Islam;Shohag, Md. Jahidul Islam;Shaibu, Abdulwahab S. S.
关键词:soybean (Glycine max L; Merrill); folate; extraction; biofortification; breeding
-
Identification and Analysis of Flax Resistance Genes to Septoria linicola (Speg.) Garassini
作者:Chen, Si;Chen, Jing;Yao, Yubo;Chen, Si;Yuan, Hongmei;Yang, Xue;Chen, Jing;Liu, Yan;Wu, Linlin;Hu, Yingying;Huang, Wengong;Yao, Yubo;Wu, Jianzhong;Kang, Qinghua;Song, Xixia;Jiang, Weidong;Sheng, Wanmin;Wu, Guangwen;Chen, Le;Wu, Guangwen
关键词:Flax; pasmo; resistance-related genes; biological information analysis; qRT-PCR; levels of expression
-
A fijiviral nonstructural protein triggers cell death in plant and bacterial cells via its transmembrane domain
作者:Yuan, Zhengjie;Geng, Yanfei;Dai, Yuanxing;Li, Jing;Lv, Mingfang;Zhang, Heng-Mu;Dai, Yuanxing;Liao, Qiansheng;Xie, Li
关键词:cell death; fijivirus; transmembrane domain; viral nonstructural protein
-
The nutritional composition of the vegetable soybean (maodou) and its potential in combatting malnutrition
作者:Agyenim-Boateng, Kwadwo Gyapong;Zhang, Shengrui;Zhang, Shibi;Khattak, Aimal Nawaz;Shaibu, Abdulwahab;Abdelghany, Ahmed M.;Qi, Jie;Azam, Muhammad;Ma, Caiyou;Feng, Yue;Feng, Huoyi;Liu, Yitian;Li, Jing;Li, Bin;Sun, Junming
关键词:soybean (Glycine max L; Merrill); maodou; macronutrients; micronutrients; malnutrition
-
Lightweight Network Ensemble Architecture for Environmental Perception on the Autonomous System
作者:Dai, Yingpeng;Wang, Junzheng;Li, Jing;Meng, Lingfeng;Wang, Songfeng
关键词:Neural network ensemble; real-time application; classification; semantic segmentation
-
Residue levels and risk assessment of pesticides in litchi and longan of China
作者:Kuang, Lixue;Wang, Zhiqiang;Cheng, Yang;Li, Yinping;Li, Haifei;Zhang, Jianyi;Shen, Youming;Li, Jing;Xu, Guofeng;Kuang, Lixue;Cheng, Yang;Li, Yinping;Li, Haifei;Zhang, Jianyi;Shen, Youming;Li, Jing;Xu, Guofeng;Kuang, Lixue;Cheng, Yang;Li, Yinping;Li, Haifei;Zhang, Jianyi;Shen, Youming;Li, Jing;Xu, Guofeng;Xu, Guofeng
关键词:Litchi; Longan; Food analysis; Pesticide residue; Risk assessment; UPLC -MS/MS; GC
-
Comparative characterization of flavor precursors and volatiles of Taihe black-boned silky fowl and Hy-line Brown yolks using multiomics and GC-O-MS-based volatilomics
作者:Yang, Weifang;Yang, Youyou;Li, Jing;Cui, Huanxian;Tang, Chaohua;Zhao, Qingyu;Jia, Yaxiong;Qin, Yuchang;Zhang, Junmin;Yang, Weifang;Wang, Liang;Lv, Xueze
关键词:Egg yolk; Flavor; Volatile compound; Lipidomics; Metabolomics; Electronic nose; Electronic tongue