Selection and validation of internal control genes for quantitative real-time RT-qPCR normalization of Phlebopus portentosus gene expression under different conditions
文献类型: 外文期刊
第一作者: Hu, Chen-Menghui
作者: Hu, Chen-Menghui;Zhou, Chen-Li;Wan, Jia-Ning;Guo, Ting;Tan, Qi;Bao, Da-Peng;Yang, Rui-Heng;Ji, Guang-Yan;Luo, Shun-Zhen;Ji, Kai-Ping;Cao, Yang
作者机构:
期刊名称:PLOS ONE ( 影响因子:3.7; 五年影响因子:3.8 )
ISSN: 1932-6203
年卷期: 2023 年 18 卷 9 期
页码:
收录情况: SCI
摘要: Phlebopus portentosus (Berk. and Broome) Boedijn is an attractive edible mushroom and is considered the only bolete for which artificial cultivation in vitro has been achieved. Gene expression analysis has become widely used in research on edible fungi and is important for elucidating the functions of genes involved in complex biological processes. Selecting appropriate reference genes is crucial to ensuring reliable RT-qPCR gene expression analysis results. In our study, a total of 12 candidate control genes were selected from 25 traditional housekeeping genes based on their expression stability in 9 transcriptomes of 3 developmental stages. These genes were further evaluated using geNorm, NormFinder, and RefFinder under different conditions and developmental stages. The results revealed that MSF1 domain-containing protein (MSF1), synaptobrevin (SYB), mitogen-activated protein kinase genes (MAPK), TATA-binding protein 1 (TBP1), and SPRY domain protein (SPRY) were the most stable reference genes in all sample treatments, while elongation factor 1-alpha (EF1), actin and ubiquitin-conjugating enzyme (UBCE) were the most unstably expressed. The gene SYB was selected based on the transcriptome results and was identified as a novel reference gene in P. portentosus. This is the first detailed study on the identification of reference genes in this fungus and may provide new insights into selecting genes and quantifying gene expression.
分类号:
- 相关文献
作者其他论文 更多>>
-
CoP-Fe2O3/g-C3N4 photocathode enhances the microbial electrosynthesis of polyhydroxybutyrate production via CO2 reduction
作者:Zhang, Kang;Xu, Xingchao;Li, Xiang Ling;Song, Tianshun;Xie, Jingjing;Zhang, Kang;Xu, Xingchao;Li, Xiang Ling;Song, Tianshun;Xie, Jingjing;Xie, Jingjing;Guo, Ting
关键词:Microbial electrosynthesis; Photoelectrocatalytic; PHB
-
Constructing carbon-based materials loaded with MOFs to realize efficient anaerobic digestion of rural organic waste
作者:Ye, Xiaomei;Jia, Zhaoyan;Liu, Yang;Wang, Chengcheng;Cao, Chunhui;Zhang, Yingpeng;Han, Ting;Wang, Li;Guo, Ting;Xi, Yonglan;Ye, Xiaomei;Jia, Zhaoyan;Liu, Yang;Wang, Chengcheng;Cao, Chunhui;Zhang, Yingpeng;Han, Ting;Wang, Li;Guo, Ting;Xi, Yonglan;Ye, Xiaomei;Jia, Zhaoyan;Liu, Yang;Wang, Chengcheng;Cao, Chunhui;Zhang, Yingpeng;Han, Ting;Wang, Li;Guo, Ting;Xi, Yonglan;Wang, Chengcheng;Cao, Chunhui;Xi, Yonglan;Xi, Yonglan
关键词:Anaerobic digestion; Metal-organic frameworks; Ammonia nitrogen inhibition; CSTR; Rural organic waste
-
Proteome changes of dairy calves rumen epithelium from birth to postweaning
作者:Zheng, Kaizhi;Wu, Jianliang;Cao, Yang;Jiang, Yongqing;Huang, Xin;Jiang, Junfang;Ullah, Saif
关键词:proteome; dairy calves; rumen; epithelium; postnatal development
-
Lysine and threonine supplementation promotes postharvest quality of Volvariella volvacea mushroom during low-temperature preservation via maintenance of the citrate cycle
作者:Zheng, Tingting;Wang, Ying;Li, Zhengpeng;Bao, Dapeng;Tan, Qi;Zou, Gen;Su, Erzheng;Zhang, Jianguo;Li, Nanyi;Wei, Yongjun;Bao, Dapeng
关键词:Volvariella volvacea; Energy metabolism; Functional food; L -lysine; L -threonine
-
Incorporating genome-wide and transcriptome-wide association studies to identify genetic elements of longissimus dorsi muscle in Huaxi cattle
作者:Liang, Mang;An, Bingxing;Deng, Tianyu;Du, Lili;Li, Keanning;Cao, Sheng;Du, Yueying;Xu, Lingyang;Zhang, Lupei;Gao, Xue;Li, Junya;Gao, Huijiang;Cao, Yang;Zhao, Yuming
关键词:longissimus dorsi muscle; GWAS; TWAS; FCT; Huaxi cattle
-
Targeted LC-MS/MS profiling of bile acids reveals primary/secondary bile acid ratio as a novel biomarker for necrotizing enterocolitis
作者:Gao, Tingting;Xu, Weijue;Wang, Zhiru;Guo, Ting;Chen, Feng;Ma, Yingxuan;Zhu, Linlin;Chen, Faling;Zhou, Jin;Lv, Zhibao;Lu, Li;Hu, Shaohua;Wang, Xiaomei
关键词:Bile acid metabolism; Primary/secondary bile acid ratio; Premature infants; Necrotizing enterocolitis
-
Multi-stage nuclear transcriptomic insights of morphogenesis and biparental role changes in Lentinula edodes
作者:Song, Xiaoxia;Chen, Mingjie;Zhao, Yan;Zhang, Meiyan;Zhang, Lujun;Zhang, Dang;Song, Chunyan;Shang, Xiaodong;Tan, Qi;Song, Chunyan
关键词:Lentinula edodes sensu lato; Genotype; Ontogeny; Parental genetic effects; Nuclear transcriptome