Label-Free Fluorometric Method for Monitoring Conformational Flexibility of Laccase Based on a Selective Laccase Sensor
文献类型: 外文期刊
第一作者: Qiu, Suyan
作者: Qiu, Suyan;Zhou, Yaomin;Li, Ruili;Zhang, Jinyan;Zhang, Dawen;Luo, Linguang;Lin, Zhenyu;Guo, Longhua;Qiu, Bin;Chen, Guonan
作者机构:
期刊名称:ANALYTICAL CHEMISTRY ( 影响因子:6.986; 五年影响因子:6.755 )
ISSN: 0003-2700
年卷期: 2013 年 85 卷 22 期
页码:
收录情况: SCI
摘要: A facile and selective fluorescence sensor for laccase determination has been proposed depending on the interaction between 3-azidocoumarin and trametes versicolor (Tv) laccase in this paper. The azido group of 3-azidocoumarin that is electron-rich alpha-nitrogen can directly interact with histidines that coordinate to three copper sites through hydrogen bonds and forms a new complex, which decreases the electron-donating ability of the azido group, leading to enhance the fluorescence intensity of the sensing system. Also, other common proteins have no significant interference for the proposed laccase sensor. Additionally, the proposed fluorescence sensor is extended to demonstrate the conformational flexibility of Tv laccase by the urea denaturant. A good consistency of the results obtained with the presented laccase sensor and CD spectra is performed. Furthermore, the relationship between the catalytic activity and the unfolding percentage of the unfolded Tv laccase through the proposed laccase sensor is also elucidated well.
分类号:
- 相关文献
作者其他论文 更多>>
-
AIM1-dependent high basal salicylic acid accumulation modulates stomatal aperture in rice
作者:Xu, Lei;Zhao, Hongyu;Jia, Xianqing;Wang, Long;Xu, Zhuang;Li, Ruili;Yi, Keke;Wang, Junbin;Xie, Xiaodong;Wang, Junbin;Wang, Xuming;Jiang, Kun;Jiang, Kun;Chen, Zhixiang;Luo, Jie
关键词:OsAIM1; rice; salicylic acid biosynthesis; shoot temperature; stomata
-
High-Performance Fluorescent Microspheres Based on Fluorescence Resonance Energy Transfer Mode for Lateral Flow Immunoassays
作者:Wang, Yumeng;Zhang, Gan;Xiao, Xiaoyue;Shu, Xinhui;Lai, Weihua;Fei, Dan;Guang, Yelan;Zhou, Yaomin
关键词:
-
Sensitive detection of cadmium ions based on a quantum-dot-mediated fluorescent visualization sensor
作者:Ai, Qiushuang;Dong, Yifan;Yu, Xiren;Zhang, Dawen;Qiu, Suyan;Wei, Peiling
关键词:
-
Pharmacokinetics, withdrawal time, and dietary risk assessment of enrofloxacin and its metabolite ciprofloxacin, and sulfachloropyridazine-trimethoprim in Taihe black-boned silky fowls
作者:Yuan, Lijuan;Zhang, Li;Xiang, Jianjun;Liao, Qiegen;Luo, Linguang;Zhang, Dawen;Wu, Huizhen;Wang, Jianmei;Zhou, Min;Qian, Mingrong
关键词:antibiotics; dietary risk assessment; pharmacokinetics; Taihe black-boned silky fowl; withdrawal time
-
Accumulation characteristics and evaluation of heavy metals in soils and vegetables of plastic-covered sheds in typical red soil areas of China
作者:Nie, Genxin;Tu, Tianhua;Hu, Lifang;Zhou, Yaomin;Nie, Genxin;Tu, Tianhua;Hu, Lifang;Zhou, Yaomin;Wu, Ling
关键词:plastic-covered shed; red soil; vegetable; heavy metal accumulation; evaluation
-
Tissue residue distribution and withdrawal time estimation of trimethoprim and sulfachloropyridazine in Yugan black-bone fowl (Gallus gallus domesticus Brisson)
作者:Zhao, Lin;Lan, Jing;Sun, Xiangdong;Wang, Bing;Ni, Bei;Wu, Shuang;Zhang, Ruiying;Liao, Hui;Zhang, Dawen;Zhang, Yun
关键词:Trimethoprim; sulfachloropyridazine; Yugan black-bone fowl; tissue residue; Withdrawal time; >
-
Effective Extraction of Bisphenol Compounds from Milk with Stable Zr(IV)-Based Metal-Organic Framework Particles
作者:Li, Tong;Wang, Ruiguo;Du, Qiuling;Cheng, Jie;Wang, Peilong;Yin, Ruijie;Xu, Hongyan;Han, Xiaoxu;Lin, Zhenyu
关键词:bisphenol compounds; metal-organic frameworks; milk; extraction