Rapid mapping of candidate genes for cold tolerance in Oryza rufipogon Griff. by QTL-seq of seedlings
文献类型: 外文期刊
第一作者: Luo Xiang-dong
作者: Luo Xiang-dong;Liu Jian;Zhao Jun;Dai Liang-fang;Chen Ya-ling;Zhang Ling;Zhang Fan-tao;Xie Jian-kun;Hu Biao-lin
作者机构:
关键词: common wild rice;cold tolerance;QTL-seq;single nucleotide polymorphisms (SNP)
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )
ISSN: 2095-3119
年卷期: 2018 年 17 卷 2 期
页码:
收录情况: SCI
摘要: Cold stress is a major problem in rice production. To rapidly identify genes for cold tolerance in Dongxiang wild rice (DWR, Oryza rufipogon Griff.), sequencing-based bulked segregant analysis of QTL-seq method was used to resequence the extremely resistant (R) and susceptible (S) bulks of a backcross inbred lines (BILs) population (derived from Oryza sativaxO. rufipogon) and their parents. Single nucleotide polymorphisms (SNP)-index graphs and corresponding Delta(SNP-index) graphs (at 99 and 95% confidence levels) for R-and S-bulks detected a total of 2 609 candidate SNPs, including 58 candidate cold-tolerance genes. Quantitative real-time PCR analysis revealed that 5 out of the 58 candidate genes had significant differences in expression between O. sativa and O. rufipogon. Structural variation and functional annotations of the 5 candidate genes were also analyzed, and allowed us to identify 2 insertion-deletion (InDel) markers (12-7 and 12-16) that were linked with candidate genes on chromosome 12 in DWR. These results are helpful for cloning and using cold tolerance genes from common wild rice in cultivated rice.
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