Development of lepidopteran pest-resistant transgenic japonica rice harboring a synthetic cry2A* gene
文献类型: 外文期刊
第一作者: Jin Yong-mei
作者: Jin Yong-mei;Ma Rui;Yu Zhi-jing;Lin Xiu-feng;Wang Ling;Jiang Wen-zhu
作者机构:
关键词: insect resistance;Bt gene;cry2A*transgenic rice;japonica rice variety;striped stem borer
期刊名称:JOURNAL OF INTEGRATIVE AGRICULTURE ( 影响因子:2.848; 五年影响因子:2.979 )
ISSN: 2095-3119
年卷期: 2015 年 14 卷 3 期
页码:
收录情况: SCI
摘要: A synthetic cry2A* gene encoding Bacillus thuringiensis (Bt) delta-endotoxin that resistance to lepidopteran pest was transformed into japonica rice variety Jijing 88, which is the most widely cultivated variety in Jilin Province, Northeast China, by Agrobacterium-mediated transformation. A total of 106 independent transformants overexpressing cry2A* gene driven by ubiquitin (Ubi) promoter was produced. Three single-copy homozygous transgenic lines were finally selected based on the results of PCR analysis, segregation ratio of Basta resistance, and Southern hybridization analyses. RT-PCR and enzyme linked immune sorbent assay (ELISA) revealed that cry2A* transcripts and protein were highly expressed in these lines. The high level of Cry2A* protein expression resulted in high resistance to rice striped stem borer as evidenced by insect feeding bioassays. Our results demonstrate that cry2A* transgenic japonica rice confers resistance to the rice striped stem borer in the laboratory conditions.
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