Immunogenicity and virus-like particle formation of rotavirus capsid proteins produced in transgenic plants
文献类型: 外文期刊
第一作者: Yang YanMei
作者: Yang YanMei;Fang RongXiang;Chen XiaoYing;Yang YanMei;Fang RongXiang;Chen XiaoYing;Yang YanMei;Li Xia;Yang Hui;Qian Yuan;Zhang You
作者机构:
关键词: rotavirus;virus-like particles;transgenic plant;oral vaccine;immunogenicity
期刊名称:SCIENCE CHINA-LIFE SCIENCES ( 影响因子:6.038; 五年影响因子:4.754 )
ISSN: 1674-7305
年卷期: 2011 年 54 卷 1 期
页码:
收录情况: SCI
摘要: The human pathogen, group A rotavirus, is the most prevalent cause of acute infantile and pediatric gastroenteritis worldwide, especially in developing countries. There is an urgent demand for safer, more effective and cheaper vaccines against rotavirus infection. Plant-derived antigens may provide an exclusive way to produce economical subunit vaccines. Virus-like particles, constituting viral capsid proteins without viral nucleic acids, are considered a far safer candidate compared with live attenuated viral vaccines. In this study, the rotavirus capsid proteins VP2, VP6 and VP7 were co-expressed in transgenic tobacco plants, and their expression levels, formation of rotavirus-like particles (RV VLPs) and immunogenicity were extensively studied. Quantitative real-time RT-PCR and Western blot analysis revealed that the expression level of vp6 was the highest while vp7 was expressed at the lowest levels. The RV VLPs were purified from transgenic tobacco plants and analyzed by electron microscopy and Western blot. Results indicated that the plant-derived VP2, VP6 and VP7 proteins self-assembled into 2/6 or 2/6/7 RV VLPs with a diameter of 60-80 nm. When orally delivered into mice with cholera toxin as an adjuvant, the total soluble protein extracted from transgenic tobacco plants induced rotavirus-specific antibodies comparable with those of attenuated rotavirus vaccines, while VP 2/6/7 induced higher serum IgG and fecal IgA titers compared with VP 2/6.
分类号:
- 相关文献
作者其他论文 更多>>
-
Study on Qualitative and Quantitative Detection of Pefloxacin and Fleroxacin Veterinary Drugs Based on THz-TDS Technology
作者:Cao Yao-yao;Li Xia;Cao Yao-yao;Bai Jun-peng;Xu Wei;Dong Chuang;Li Bin;Ni Ying;Cao Yao-yao;Bai Jun-peng;Xu Wei;Dong Chuang;Li Bin;Zhong Hong-li
关键词:THz-TDS; Pefloxacin; Fleroxacin; Qualitative discrimination; Quantitative detection
-
Transcriptomic and metabolomic insights into the adaptive response of Salix viminalis to phenanthrene
作者:Li Xia;Ma Xiaodong;Liu Junxiang;Zou Junzhu;Sun Zhenyuan;Han Lei;Li Xia;Cheng Yunhe;Zhai Feifei
关键词:Defensive mechanisms; Metabolic pathways; Phenanthrene; Salix viminalis
-
Genome-Wide Identification of Zn(2)Cys(6) Class Fungal-Specific Transcription Factors (ZnFTFs) and Functional Analysis of UvZnFTF1 in Ustilaginoidea virens
作者:Song Tianqiao;Zhang You;Liang Dong;Yan Jiaoling;Yu Junjie;Yu Mina;Cao Huijuan;Yong Mingli;Pan Xiayan;Qi Zhongqiang;Du Yan;Zhang Rongsheng;Liu Yongfeng;Song Tianqiao;Zhang Xiong;Liu Yongfeng
关键词:Zn(2)Cys(6)-type fungal-specific transcription factor; Ustilaginoidea virens; rice pathogen; gene silencing; RNA-Seq; metabolism pathway; effector expression; pathogen-host interaction
-
Sensitivity of rice to photooxidation associated with antioxidant enzyme activity and carbon assimilation
作者:Zeng Yanda;Zhang Qijun;Xiao Enxing;Li Xia;Lv Chuangen;Zhang Qijun;Li Xia;Lv Chuangen
关键词:Oryza sativa L; Photooxidation; Chlorophyll fluorescence parameter; Antioxidant enzyme activity; RNA-seq; Gene expression
-
Intake of Diacylglycerols and the Fasting Insulin and Glucose Concentrations: A Meta-Analysis of 5 Randomized Controlled Studies
作者:Xu Tongcheng;Jia Min;Qiu Bin;Liu Wei;Zong Aizhen;Liu Lina;Du Fangling;Li Xia;Zhang Yuan
关键词:Diacylglycerols; insulin; glucose; meta-analysis; diabetes
-
Economic efficiency of small-scale tilapia farms in Guangxi, China
作者:Zhang Zongli;Zhang Yanan;Li Feifan;Yang Hui;Yuan Yongming;Yuan Xinhua;Zhang Zongli;Li Feifan;Yuan Yongming;Yuan Xinhua
关键词:Allocative efficiency; data envelopment analysis; economic efficiency; small-scale tilapia farm; technical efficiency; tobit regression
-
Experimental study on performance of pneumatic seeding system
作者:Liu Lijing;Liu Lijing;Yang Hui;Ma Shaochun
关键词:pneumatic seeding system;seeding rate;air flow rate;quadratic general rotary unitized design;regression model