Comparative functional analysis of macrophage phagocytosis in Dagu chickens and Wenchang chickens
文献类型: 外文期刊
第一作者: Zhang, Jin
作者: Zhang, Jin;Wang, Qiao;Li, Qinghe;Wang, Zixuan;Zheng, Maiqing;Wen, Jie;Zhao, Guiping
作者机构:
关键词: macrophage; phagocytosis; chicken; Salmonnella; transcriptomics; genomic selection
期刊名称:FRONTIERS IN IMMUNOLOGY ( 影响因子:7.3; 五年影响因子:8.0 )
ISSN: 1664-3224
年卷期: 2023 年 14 卷
页码:
收录情况: SCI
摘要: Phagocytosis of macrophages constitutes a powerful barrier to innate immunity. Differences in the phagocytic function of macrophages among chicken breeds have rarely been reported, and the molecular mechanisms underlying phagocytosis remain poorly understood. This study compared functional difference of macrophages in Dagu chickens, originated in Zhuanghe, Liaoning Province, China, and Wenchang chickens, originated from Hainan Island in the South China Sea, and explored the potential molecular mechanisms by integrated analysis of mRNA expression profiles of macrophages and whole genome sequencing. Immunological parameters in peripheral blood indicated that Dagu chickens were more resistant to Salmonella challenge at 28 days old. Phagocytosis index and phagocytosis rate of macrophages displayed Dagu chickens performed a significantly higher phagocytic ability of macrophages at 14 and 28 days old. Furthermore, comparative analysis of mRNA expression profiles of macrophages of two breeds at 28 days old revealed that 1136 differentially expressed genes (DEGs), and 22 DEGs (e.g., H2AFZ, SNRPA1, CUEDC2, S100A12) were found to be hub genes regulating phagocytosis by participating in different immunological biological signaling pathways. In addition, many DEGs and hub genes were under strong differentiation in genome between two breeds, the H2AFZ gene was an intersection of DEGs and hub genes. These results provided a comprehensive functional comparison and transcriptomic profiles of macrophages in Chinese native chicken breeds, and deepened our understanding of the genetic mechanism of innate immunity.
分类号:
- 相关文献
作者其他论文 更多>>
-
Proximate composition and nutritional analysis of selected bananas cultivated in Hainan, China
作者:Tan, Lin;He, Yingdui;Solis-Sainz, Juan Carlos;Li, Sunjing;Deng, Jie;Avula, Bharathi;Zhang, Jin;Pugh, Nirmal D.;Katragunta, Kumar;Solis-Sainz, Juan Carlos;Wang, Mei
关键词:Bananas; Proximate composition; Vitamin C and B6; Free amino acids; Minerals; Bioactive compounds
-
miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene
作者:Zhou, Kai-Zhi;Wu, Peng-Fei;Ling, Xuan-Ze;Zhang, Jin;Wang, Qi-Fan;Zhang, Xin-Chao;Zhang, Tao;Zhang, Gen-Xi;Xue, Qian;Han, Wei
关键词:miRNA; skeletal muscle; growth and development; chicken
-
miR-375 upregulates lipid metabolism and inhibits cell proliferation involved in chicken fatty liver formation and inheritance via targeting recombination signal binding protein for immunoglobulin kappa J region (RBPJ)
作者:Zhang, Yong-Hong;Xie, Heng-Li;Yang, Yu-Wei;Zhang, Jia-Bao;Wen, Jie;Liu, Ran-Ran;Zhao, Gui-Ping;Tan, Xiao-Dong;Liu, Zhen;Zheng, Yi
关键词:chicken; fatty liver; miR-375; RBPJ; lipid metabolism
-
Effects of dietary supplementation with dandelion tannins or soybean isoflavones on growth performance, antioxidant function, intestinal morphology, and microbiota composition in Wenchang chickens
作者:Li, Xiang;Sun, Ruiping;Ou, Yangkun;Qi, Qi;Xie, Yali;Wei, Limin;Li, Xiang;Gong, Yuanfang;Liu, Quanwei;Zhao, Guiping;Wei, Limin;Wang, Xiuping;Hu, Chenjun;Jiang, Shouqun
关键词:growth performance; biochemical blood indexes; antioxidant function; intestinal health; dandelion tannin; soybean isoflavones; Wenchang chicken
-
Development of a TaqMan-Probe-Based Multiplex Real-Time PCR for the Simultaneous Detection of Porcine Circovirus 2, 3, and 4 in East China from 2020 to 2022
作者:Zou, Jianwen;Liu, Huaicheng;Chen, Jing;Zhang, Jin;Li, Xiaohan;Zhou, Bin;Long, Yunfeng;Jiang, Yan;Li, Wenliang
关键词:porcine circovirus (PCV); real-time qPCR; TaqMan probe; differential diagnosis; multiple infection
-
Fructose-enabled killing of antibiotic-resistant Salmonella enteritidis by gentamicin: Insight from reprogramming metabolomics
作者:Jiang, Ming;Xie, Chun-Lin;Chen, Peng;Luo, Wei;Lin, Chu-xiao;Shu, Ding-Ming;Luo, Cheng-long;Qu, Hao;Ji, Jian;Jiang, Ming;Li, Xia;Wang, Qiao;Qu, Hao;Ji, Jian
关键词:Salmonella enterica; Aminoglycoside antibiotics; Metabolomics; Antibiotic resistance; Chicken
-
Selection of goat 8-casein derived ACE-inhibitory peptide SQPK and insights into its effect and regulatory mechanism on the function of endothelial cells
作者:Wu, Yulong;Zhang, Jin;Mu, Tong;Zhang, Hong;Cao, Jianxin;Li, Huanhuan;Tang, Honggang;Chen, Lihong;Zhao, Ke;Wu, Yulong;Xu, Xianrong;Liu, Hongyun;Zhao, Ke
关键词:SQPK; ACE inhibitory peptide; Goat 8-casein; Endothelial cells; Transcriptomic profiling