Cloning, ligand-binding, and temporal expression of ecdysteroid receptors in the diamondback moth, Plutella xylostella

文献类型： 外文期刊

第一作者： Tang, Baozhen

作者： Tang, Baozhen;Dong, Wei;Liang, Pei;Gao, Xiwu;Dong, Wei;Zhou, Xuguo

作者机构：

关键词： Plutella xylostella;Ecdysone receptor (EcR);Binding affinity;Expression profiling;Ecdysone agonist

期刊名称：BMC MOLECULAR BIOLOGY （ 影响因子：2.946； 五年影响因子：3.192 ）

ISSN： 1471-2199

年卷期： 2012 年 13 卷

页码：

收录情况： SCI

摘要： Background: The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a devastating pest of cruciferous crops worldwide, and has developed resistance to a wide range of insecticides, including diacylhydrazine-based ecdysone agonists, a highly selective group of molt-accelerating biopesticides targeting the ecdysone receptors. Result: In this study, we cloned and characterized the ecdysone receptors from P. xylostella, including the two isoforms of EcR and a USP. Sequence comparison and phylogenetic analysis showed striking conservations among insect ecdysone receptors, especially between P. xylostella and other lepidopterans. The binding affinity of ecdysteroids to in vitro-translated receptor proteins indicated that PxEcRB isoform bound specifically to ponasterone A, and the binding affinity was enhanced by co-incubation with PxUSP (K-d = 3.0 +/- 1.7 nM). In contrast, PxEcRA did not bind to ponasterone A, even in the presence of PxUSP. The expression of PxEcRB were consistently higher than that of PxEcRA across each and every developmental stage, while the pattern of PxUSP expression is more or less ubiquitous. Conclusions: Target site insensitivity, in which the altered binding of insecticides (ecdysone agonists) to their targets (ecdysone receptors) leads to an adaptive response (resistance), is one of the underlying mechanisms of diacylhydrazine resistance. Given the distinct differences at expression level and the ligand-binding capacity, we hypothesis that PxEcRB is the ecdysone receptor that controls the remodeling events during metamorphosis. More importantly, PxEcRB is the potential target site which is modified in the ecdysone agonist-resistant P. xylostella.

分类号：

  • 相关文献

[1]RNA Interference of the Ecdysone Receptor Genes EcR and USP in Grain Aphid (Sitobion avenae F.) Affects Its Survival and Fecundity upon Feeding on Wheat Plants. Yan, Ting,Chen, Hongmei,Sun, Yongwei,Yu, Xiudao,Xia, Lanqin. 2016

[2]Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Xu, Jian,Wu, Jing,Jiang, Bo,Zhang, Xixi,Li, Xiaoyang,Yang, Dawei,Huang, Xiufen,Li, Yongqing,Wu, Jing,He, Houjun,Li, Xiaoyang,Zhang, Xixi,Sealy, Joshua E.. 2017

[3]Toxicity and binding analyses of Bacillus thuringiensis toxin Vip3A in Cry1Ac-resistant and -susceptible strains of Helicoverpa armigera (Hubner). Lu Qiong,Zhang Yan,Liang Ge-mei,Zhang Qian,Chen Li-zhen. 2015

[4]New insights on the role of alkaline phosphatase 2 from Spodoptera exigua (Hubner) in the action mechanism of Bt toxin Cry2Aa. Zhao, Man,Wei, Jizhen,Zhang, Wanna,Wang, Bingjie,Khaing, Myint Myint,Liang, Gemei.

[5]Molecular cloning, expression profile, odorant affinity, and stability of two odorant-binding proteins in Macrocentrus cingulum Brischke (Hymenoptera: Braconidae). Zhang, Tiantao,Wang, Zhenying,He, Kanglai,Bai, Shuxiong,Ahmed, Tofael.

[6]Functional genomics to study stress responses in crop legumes: progress and prospects. Kudapa, Himabindu,Ramalingam, Abirami,Nayakoti, Swapna,Varshney, Rajeev K.,Ramalingam, Abirami,Chen, Xiaoping,Liang, Xuanqiang,Varshney, Rajeev K.,Zhuang, Wei-Jian,Kahl, Guenter,Kahl, Guenter,Edwards, David,Varshney, Rajeev K.. 2013

[7]Genome-wide analysis of the TPX2 family proteins in Eucalyptus grandis. Gan, Siming,Du, Pingzhou,Yao, Yuan,Wang, Yuqi,Du, Pingzhou,Yao, Yuan,Xie, Qiaoli,Wu, Ai-Min,Du, Pingzhou,Yao, Yuan,Xie, Qiaoli,Wu, Ai-Min,Du, Pingzhou,Wang, Jinyan,Zhang, Baolong,Kumar, Manoj,Wang, Yuqi. 2016

[8]Identification, bioinformatic analysis and expression profiling of candidate mRNA-like non-coding RNAs in Sus scrofa. Xiao, Bang,Zhang, Xingju,Li, Yong,Tang, Zhonglin,Yang, Shulin,Mu, Yulian,Cui, Wentao,Ao, Hong,Li, Kui,Xiao, Bang. 2009

[9]A novel functional gene associated with cold tolerance at the seedling stage in rice. Zhao, Junliang,Zhang, Shaohong,Dong, Jingfang,Yang, Tifeng,Mao, Xingxue,Liu, Qing,Wang, Xiaofei,Liu, Bin,Zhao, Junliang,Zhang, Shaohong,Dong, Jingfang,Yang, Tifeng,Mao, Xingxue,Liu, Qing,Wang, Xiaofei,Liu, Bin. 2017

[10]Peanut (Arachis hypogaea L.) Omics and Biotechnology in China. Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Wang, Xing-Jun,Xia, Han,Wan, Shu-Bo,Zhao, Chuan-Zhi,Li, Ai-Qin,Liu, Shuan-Tao. 2011

[11]Genome-wide analysis of the Solanum tuberosum (potato) trehalose-6-phosphate synthase (TPS) gene family: evolution and differential expression during development and stress. Xu, Yingchun,Wang, Yanjie,Jin, Qijiang,Mattson, Neil,Yang, Liu. 2017

[12]Genome-wide identification and evolutionary analysis of leucine-rich repeat receptor-like protein kinase genes in soybean. Qiu, Li-Juan. 2016

[13]Identification and characterization of the zinc-regulated transporters, iron-regulated transporter-like protein (ZIP) gene family in maize. Li, Suzhen,Huang, Yaqun,Zhu, Liying,Zhao, Yongfeng,Guo, Jinjie,Chen, Jingtang,Li, Suzhen,Zhou, Xiaojin,Zhang, Shaojun,Chen, Rumei. 2013

[14]Genome-wide identification, classification and expression profiling of nicotianamine synthase (NAS) gene family in maize. Zhou, Xiaojin,Zhao, Qianqian,Liu, Xiaoqing,Zhang, Shaojun,Sun, Cheng,Fan, Yunliu,Zhang, Chunyi,Chen, Rumei,Zhou, Xiaojin,Zhao, Qianqian,Liu, Xiaoqing,Zhang, Shaojun,Sun, Cheng,Fan, Yunliu,Zhang, Chunyi,Chen, Rumei,Li, Suzhen. 2013

[15]Gene and protein expression profiling analysis of young spike development in large spike wheat germplasms. Chen Dan,Zhang Jin-peng,Liu Wei-hua,Wu Xiao-yang,Yang Xin-ming,Li Xiu-quan,Lu Yu-qing,Li Li-hui,Chen Dan. 2016

[16]Transcript profiling of a dominant male sterile mutant (Ms-cd1) in cabbage during flower bud development. Lou, Ping,Kang, Jungen,Zhang, Guoyu,Bonnema, Guusje,Fang, Zhiyuan,Wang, Xiaowu. 2007

[17]Genome-wide analysis of WRKY gene family in the sesame genome and identification of the WRKY genes involved in responses to abiotic stresses. Li, Donghua,Liu, Pan,Yu, Jingyin,Wang, Linhai,Dossa, Komivi,Zhang, Yanxin,Zhou, Rong,Wei, Xin,Zhang, Xiurong,Dossa, Komivi,Wei, Xin. 2017

[18]Identification and Characterization of 12 Mitogen-activated Protein Kinase Genes Implicated in Stress Responses in Cherry Rootstocks. Zong, Xiaojuan,Wang, Jiawei,Xu, Li,Wei, Hairong,Chen, Xin,Zhu, Dongzi,Tan, Yue,Liu, Qingzhong.

[19]Genome-wide transcriptome analysis of two maize inbred lines under drought stress. Zheng, Jun,Liu, Yunjun,Wang, Guoying,Zheng, Jun,Liu, Yunjun,Wang, Guoying,Fu, Junjie,Gou, Mingyue,Huai, Junling,Jian, Min,Guo, Xiying,Dong, Zhigang,Wang, Guoying,Huang, Quansheng,Wang, Hongzhi.

[20]Genome-wide characterization and expression profiling of the NAC genes under abiotic stresses in Cucumis sativus. Zhang, Xiao Meng,Yu, Hong Jun,Sun, Chao,Deng, Jie,Zhang, Xue,Liu, Peng,Li, Yun Yun,Li, Qiang,Jiang, Wei Jie,Sun, Chao,Jiang, Wei Jie.

作者其他论文 更多>>

微信小程序