Glucometer-based biosensor for the determination of ractopamine in animal-derived foods using rolling circle amplification
文献类型: 外文期刊
第一作者: He, Feng
作者: He, Feng;Wang, Haijie;Du, Pengfei;Wang, Weiting;Liu, Yaobo;Wang, Shoujing;Ma, Yanli;Wang, Yuanshang;Hu, Peng;He, Feng;Li, Tengfei;Tan, Tianyu;Wang, Haijie;Wang, Yuanshang;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Abd El-Aty, A. M.
作者机构:
关键词: Ractopamine; Rolling circle amplification; Glucometer; Gold nanoparticles; Portable sensing
期刊名称:MICROCHIMICA ACTA ( 影响因子:5.7; 五年影响因子:5.4 )
ISSN: 0026-3672
年卷期: 2023 年 190 卷 4 期
页码:
收录情况: SCI
摘要: Screening for persistent organic pollutants (POPs) in food is a complex and challenging process, as POPs can be present in very low levels and can be difficult to detect. Herein, we developed an ultrasensitive biosensor based on a rolling circle amplification (RCA) platform using a glucometer to determine POP. The biosensor was constructed using gold nanoparticle probes modified with antibodies and dozens of primers, magnetic microparticle probes conjugated with haptens, and targets. After competition, RCA reactions are triggered, numerous RCA products hybridize with the ssDNA-invertase, and the target is successfully transformed into glucose. Using ractopamine as a model analyte, this strategy obtained a linear detection range of 0.038-5.00 ng mL(-1) and a detection limit of 0.0158 ng mL(-1), which was preliminarily verified by screening in real samples. Compared with conventional immunoassays, this biosensor utilizes the high efficiency of RCA and the portable properties of a glucometer, which effectively improves the sensitivity and simplifies the procedures using magnetic separation technology. Moreover, it has been successfully applied to ractopamine determination in animal-derived foods, revealing its potential as a promising tool for POP screening.
分类号:
- 相关文献
作者其他论文 更多>>
-
The responsive mechanisms of DNA methylation and transcriptional regulation to acute hypoxia stress in HIF-1/VEGFA signal pathway of Japanese flounder (Paralichthys olivaceus)
作者:Liu, Binghua;Li, Guangling;Li, Xiaohui;Wang, Hao;Yang, Jun;Wen, Haishen;He, Feng;Liu, Binghua
关键词:DNA methylation; Transcriptional regulation; Binding site; Hypoxia stress; Signal pathway; Japanese flounder
-
Comparison of Chemiluminescence Enzyme Immunoassay (Cl-ELISA) with Colorimetric Enzyme Immunoassay (Co-ELISA) for Imidacloprid Detection in Vegetables
作者:Zhai, Rongqi;Chen, Ge;Liu, Guangyang;Huang, Xiaodong;Xu, Xiaomin;Li, Lingyun;Zhang, Yanguo;Xu, Donghui;Abd El-Aty, A. M.;Abd El-Aty, A. M.
关键词:imidacloprid; colorimetric assay; chemiluminescent assay; enzyme-linked immunoassay
-
A Novel Fluorescent Sensor Based on Aptamer and qPCR for Determination of Glyphosate in Tap Water
作者:Shao, Yong;Tian, Run;Wang, Miao;Cao, Jing;Cao, Zhen;Jin, Fen;She, Yongxin;Shao, Yong;Tian, Run;Wang, Miao;Cao, Jing;Cao, Zhen;Jin, Fen;She, Yongxin;Duan, Jiaqi;Li, Guangyue;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Abd El-Aty, A. M.
关键词:glyphosate; aptamer; qPCR; SYBR Green I; sensor
-
Application of Molecularly Imprinted Electrochemical Biomimetic Sensors for Detecting Small Molecule Food Contaminants
作者:Shao, Yunling;Wang, Miao;Cao, Jing;She, Yongxin;Cao, Zhen;Jin, Fen;Wang, Jing;Shao, Yunling;Wang, Miao;Cao, Jing;She, Yongxin;Cao, Zhen;Jin, Fen;Wang, Jing;Duan, Jiaqi;Li, Guangyue;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Abd El-Aty, A. M.
关键词:molecular imprinting; electrochemical biomimetic sensors; small-molecule chemical contaminants
-
Transcriptome and metabolome analyses reveal improvement in blueberry fruit quality by interspecific grafting
作者:Zhu, Bo;Guo, Pei-Pei;Shen, Min;Zhang, Yan;He, Feng;Yang, Lu;Gao, Xuan;Xiao, Jia-Xin;Shen, Min;Hu, Yong
关键词:Vaccinium corymbosum; Interspecific grafting; Sugar metabolism; Shikimate-derived metabolism
-
Development of a biocompatible green drug release system using salidroside-TiO2-doped chitosan oligosaccharide molecularly imprinted polymers
作者:Liu, Zijie;Ma, Xingbin;Li, Shuyu;Qiu, Jiajie;Liu, Siyu;Huang, Zhifeng;Lin, Hongling;Abd El-Aty, A. M.;Abd El-Aty, A. M.;Abd El-Aty, A. M.
关键词:Surface molecularly; imprinted polymer; Novel functional monomer; Diethylene glycol; Sustained release; Salidroside; dimethacrylate
-
MB @ ZIF-8/COOH-MWCNTs Composite-based Ratiometric Electrochemical Sensor for Detection of Hydrogen Peroxide
作者:Zhao, Si-Jia;He, Feng;Liu, Tian-Di;Zhang, Shao-Ying;Li, Teng-Fei;Wang, Jing;He, Feng;Du, Peng-Fei
关键词:Hydrogen peroxide; 4-Aminophenylboronic acid; Methylene blue; Ratiometric electrochemical sensor