Cloning and Functional Characterization of Cold-Inducible MYB-like 17 Transcription Factor in Rapeseed (Brassica napus L.)
文献类型: 外文期刊
第一作者: Luo, Dan
作者: Luo, Dan;Raza, Ali;Cheng, Yong;Zou, Xiling;Lv, Yan
作者机构:
关键词: abiotic stress; canola; gene overexpression; low temperature; osmoprotectants; transcriptome analysis
期刊名称:INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES ( 影响因子:5.6; 五年影响因子:6.2 )
ISSN: 1661-6596
年卷期: 2023 年 24 卷 11 期
页码:
收录情况: SCI
摘要: Rapeseed (Brassica napus L.) is an important crop for edible oil, vegetables, and biofuel. Rapeseed growth and development require a minimum temperature of similar to 1-3 degrees C. Notably, frost damage occurs during overwintering, posing a serious threat to the productivity and yield of rapeseed. MYB proteins are important transcription factors (TFs) in plants, and have been proven to be involved in the regulation of stress responses. However, the roles of the MYB TFs in rapeseed under cold stress conditions are yet to be fully elucidated. To better understand the molecular mechanisms of one MYB-like 17 gene, BnaMYBL17, in response to low temperature, the present study found that the transcript level of BnaMYBL17 is induced by cold stress. To characterize the gene's function, the 591 bp coding sequence (CDS) from rapeseed was isolated and stably transformed into rapeseed. The further functional analysis revealed significant sensitivity in BnaMYBL17 overexpression lines (BnaMYBL17-OE) after freezing stress, suggesting its involvement in freezing response. A total of 14,298 differentially expressed genes relative to freezing response were found based on transcriptomic analysis of BnaMYBL17-OE. Overall, 1321 candidate target genes were identified based on differential expression, including Phospholipases C1 (PLC1), FCS-like zinc finger 8 (FLZ8), and Kinase on the inside (KOIN). The qPCR results confirmed that the expression levels of certain genes showed fold changes ranging from two to six when compared between BnaMYBL17-OE and WT lines after exposure to freezing stress. Furthermore, verification indicated that BnaMYBL17 affects the promoter of BnaPLC1, BnaFLZ8, and BnaKOIN genes. In summary, the results suggest that BnaMYBL17 acts as a transcriptional repressor in regulating certain genes related to growth and development during freezing stress. These findings provide valuable genetic and theoretical targets for molecular breeding to enhance freezing tolerance in rapeseed.
分类号:
- 相关文献
作者其他论文 更多>>
-
Preparation and digestive characteristics of myricitrin-loaded nanoemulsion delivery systems with glycated soybean oil body and x-carrageenan as emulsifiers
作者:Chang, Guoli;Luo, Dan;Tian, Siyi;Cai, Chenggang;Zhu, Ruiyu;Cai, Haiying;Yang, Hailong;Gao, Haiyan;Cai, Chenggang
关键词:Myricitrin; Nanoemulsion; Soybean oil body; Glycation; Bioavailability
-
Natural resistance-associated macrophage proteins (NRAMPs) are involved in cadmium enrichment in peanut (Arachishypogaea L.) under cadmium stress
作者:Yan, Lei;Jin, Haotian;Huang, Yang;Gu, De Ping;Zou, Xiaoyun;Raza, Ali;Jin, Haotian
关键词:Food safety; Gene expression; Oilseed crop; Plant genomics; Toxic metals
-
Locking-DNA network regulated CRISPR-Cas12a fluorescent aptasensor based on hollow flower-like magnetic MoS2 microspheres for sensitive detection of sulfadimethoxine
作者:Lv, Yan;Sun, Yuhan;Khan, Imran Mahmood;Li, Qiaofeng;Yue, Lin;Wang, Zhouping;Lv, Yan;Sun, Yuhan;Khan, Imran Mahmood;Li, Qiaofeng;Yue, Lin;Wang, Zhouping;Zhang, Yin;Wang, Zhouping;Wang, Zhouping;Wang, Zhouping;Zhou, You
关键词:Flower-like Fe 3 O 4 @MoS 2 microsphere; Locking-DNA network; CRISPR-Cas12a; Aptasensor; Sulfadimethoxine
-
Role of phytohormones in regulating cold stress tolerance: Physiological and molecular approaches for developing cold-smart crop plants
作者:Raza, Ali;Charagh, Sidra;Najafi-Kakavand, Shiva;Abbas, Saghir;Shoaib, Yasira;Anwar, Sultana;Sharifi, Sara;Lu, Guangyuan;Siddique, Kadambot H. M.
关键词:Chilling stress; Climate change; CBF genes; Freezing stress; Genetic engineering; Omics; Phytohormones; Signaling cascade; Temperature changes
-
Integrating by-products from bioenergy technology to improve the morpho-physiological growth and yield of soybean under acidic soil
作者:Sattar, Muhammad Tayyab;Shen, Fei;Sattar, Muhammad Tayyab;Shen, Fei;Raza, Ali;Raza, Ali;Ali, Shafaqat;Ali, Shafaqat;Bashir, Shanzay;Kanwal, Farah;Khan, Imran;Raza, Muhammad Ali;Hussain, Sajad;Raza, Muhammad Ali
关键词:Bioenergy waste; Biomass; Photosynthesis; Soil reclamation; Soybean yield
-
Developing future heat-resilient vegetable crops
作者:Saeed, Faisal;Chaudhry, Usman Khalid;Raza, Ali;Charagh, Sidra;Bakhsh, Allah;Bohra, Abhishek;Chitikineni, Annapurna;Varshney, Rajeev K.;Ali, Sumbul;Chitikineni, Annapurna;Varshney, Rajeev K.;Saeed, Yasir;Visser, Richard G. F.;Siddique, Kadambot H. M.
关键词:Abiotic stress; Biotechnology; Climate change; Heat stress; GWAS; Genome editing; QTL mapping
-
Genome-wide identification of papain-like cysteine protease family genes in cultivated peanut (Arachis hypogaea L.) and functional characterization of AhRD21B in response to chilling stress
作者:Zhang, Chong;Chen, Yuting;Wang, Lihui;Liu, Lu;Zhong, Xin;Chu, Panpan;Gao, Meijia;Chen, Hua;Cai, Tiecheng;Raza, Ali;Zhuang, Weijian;Zhang, Chong;Chen, Yuting;Wang, Lihui;Liu, Lu;Zhong, Xin;Chu, Panpan;Gao, Meijia;Chen, Hua;Cai, Tiecheng;Raza, Ali;Zhuang, Weijian;Xiong, Faqian;Zhang, Chong;Zhang, Xiurong;Liu, Fengzhen;Pan, Rong-long;Varshney, Rajeev K.
关键词:Arachis hypogaea; PLCPs; Gene expression; AhRD21B; Cold stress