DICER-LIKE2 Plays a Crucial Role in Rice Stripe Virus Coat Protein-Mediated Virus Resistance in Arabidopsis
文献类型: 外文期刊
第一作者: Chen, Li
作者: Chen, Li;Zou, Baohong;Chen, Li;Liu, Yanan;Li, Shuo;Ji, Yinghua;Sun, Feng
作者机构:
关键词: DCL2; DCL4; rice stripe virus; CP-mediated resistance; RNA silencing; small RNA deep sequence
期刊名称:VIRUSES-BASEL ( 影响因子:4.7; 五年影响因子:4.8 )
ISSN:
年卷期: 2023 年 15 卷 11 期
页码:
收录情况: SCI
摘要: Virus coat protein (CP)-mediated resistance is considered an effective antiviral defense strategy that has been used to develop robust resistance to viral infection. Rice stripe virus (RSV) causes significant losses in rice production in eastern Asia. We previously showed that the overexpression of RSV CP in Arabidopsis plants results in immunity to RSV infection, using the RSV-Arabidopsis pathosystem, and this CP-mediated viral resistance depends on the function of DCLs and is mostly involved in RNA silencing. However, the special role of DCLs in producing t-siRNAs in CP transgenic Arabidopsis plants is not fully understood. In this study, we show that RSV CP transgenic Arabidopsis plants with the dcl2 mutant background exhibited similar virus susceptibility to non-transgenic plants and were accompanied by the absence of transgene-derived small interfering RNAs (t-siRNAs) from the CP region. The dcl2 mutation eliminated the accumulation of CP-derived t-siRNAs, including those generated by other DCL enzymes. In contrast, we also developed RSV CP transgenic Arabidopsis plants with the dcl4 mutant background, and these CP transgenic plants showed immunity to virus infection and accumulated comparable amounts of CP-derived t-siRNAs to CP transgenic Arabidopsis plants with the wild-type background except for a significant increase in the abundance of 22 nt t-siRNA reads. Overall, our data indicate that DCL2 plays an essential, as opposed to redundant, role in CP-derived t-siRNA production and induces virus resistance in RSV CP transgenic Arabidopsis plants.
分类号:
- 相关文献
作者其他论文 更多>>
-
Potential of globally distributed topsoil mid-infrared spectral library for organic carbon estimation
作者:Hong, Yongsheng;Hong, Yongsheng;Sanderman, Jonathan;Hengl, Tomislav;Chen, Songchao;Wang, Nan;Xue, Jie;Shi, Zhou;Zhuo, Zhiqing;Peng, Jie;Li, Shuo;Chen, Yiyun;Liu, Yaolin;Mouazen, Abdul Mounem;Mouazen, Abdul Mounem
关键词:Soil monitoring; Mid-infrared spectroscopy; Soil spectral library; Fractional-order derivative; Deep learning
-
Combined quantitative lipidomics and back-propagation neural network approach to discriminate the breed and part source of lamb
作者:Liu, Chongxin;Zhang, Dequan;Li, Shaobo;Liu, Chunyou;Zheng, Xiaochun;Li, Cheng;Chen, Li;Liu, Chongxin;Dunne, Peter;Brunton, Nigel Patrick;Grasso, Simona;Liu, Chunyou
关键词:Lipidomics; Lamb; Food authenticity; Linear discriminant model; Machine learning; Neural network
-
A nitrogen fixing symbiosis-specific pathway required for legume flowering
作者:Yun, Jinxia;Wang, Can;Zhang, Fengrong;Sun, Zhengxi;Luo, Yuanqing;Liao, Junwen;Wang, Yongliang;Cha, Yanyan;Zhang, Xuehai;Ren, Ya;Wu, Jun;Li, Xia;Chen, Li;Cai, Yupeng;Hou, Wensheng;Chen, Li;Cai, Yupeng;Hou, Wensheng;Han, Tianfu;Hasegawa, Paul M.;Tian, Changfu;Tian, Changfu;Tian, Changfu;Su, Huanan;Ferguson, Brett J.;Gresshoff, Peter M.;Li, Xia;Sun, Zhengxi
关键词:
-
Insights into the gel and electronic sense characteristics of meat batters made from Funiu white goat and Oula sheep meat in different rigor states
作者:Li, Shaobo;Zhang, Dequan;Xiang, Can;Ge, Yue;Liu, Huan;Zheng, Xiaochun;Chen, Li;Wang, Zhenyu;Chen, Li;Wang, Zhenyu
关键词:Meat batters; Pre-rigor meat; Post-rigor meat; Gel properties; Electronic sense
-
Non-Destructive Detection of Meat Quality Based on Multiple Spectral Dimension Reduction Methods by Near-Infrared Spectroscopy
作者:Zheng, Xiaochun;Chen, Li;Li, Xin;Zhang, Dequan
关键词:non-destructive detection; meat quality; dimension reduction; near-infrared spectroscopy
-
Integration of LC-MS-Based and GC-MS-Based Metabolic Profiling to Reveal the Effects of Domestication and Boiling on the Composition of Duck Egg Yolks
作者:Tian, Yong;Li, Guoqin;Zeng, Tao;Chen, Li;Xu, Wenwu;Gu, Tiantian;Tao, Zhengrong;Lu, Lizhi;Tian, Yong;Li, Guoqin;Zeng, Tao;Chen, Li;Xu, Wenwu;Gu, Tiantian;Lu, Lizhi;Du, Xizhong
关键词:duck egg yolk; metabolomics; domestication; boiling; lipid metabolism; stress resistance
-
Changes in planting methods will change the potential distribution of rice in South China under climate warming
作者:Li, Shuo;Zhao, Jie;Chen, Fu;Chu, Qingquan;Li, Shuo;Lu, Zhou;Luo, Ming;Chu, Qingquan
关键词:Climate warming; Direct-seeded rice; Planting methods; Distribution; Food security; South China