Artificial evolution of OsEPSPS through an improved dual cytosine and adenine base editor generated a novel allele conferring rice glyphosate tolerance
文献类型: 外文期刊
第一作者: Zhang, Chen
作者: Zhang, Chen;Zhong, Xue;Li, Shaoya;Yan, Lei;Li, Jingying;He, Yubing;Xia, Lanqin;Zhong, Xue;Li, Shaoya;Li, Jingying;He, Yubing;Xia, Lanqin;Li, Shaoya;Li, Jingying;He, Yubing;Xia, Lanqin;Lin, Yong;Zhang, Yangjun
作者机构:
关键词: artificial evolution; dual cytosine and adenine base editor; nCas9-NG; rice (Oryza sativa L); near-saturated mutagenesis
期刊名称:JOURNAL OF INTEGRATIVE PLANT BIOLOGY ( 影响因子:11.4; 五年影响因子:10.1 )
ISSN: 1672-9072
年卷期: 2023 年
页码:
收录情况: SCI
摘要: Exploiting novel endogenous glyphosate-tolerant alleles is highly desirable and has promising potential for weed control in rice breeding. Here, through fusions of different effective cytosine and adenine deaminases with nCas9-NG, we engineered an effective surrogate two-component composite base editing system, STCBE-2, with improved C-to-T and A-to-G base editing efficiency and expanded the editing window. Furthermore, we targeted a rice endogenous OsEPSPS gene for artificial evolution through STCBE-2-mediated near-saturated mutagenesis. After hygromycin and glyphosate selection, we identified a novel OsEPSPS allele with an Asp-213-Asn (D213N) mutation (OsEPSPS-D213N) in the predicted glyphosate-binding domain, which conferred rice plants reliable glyphosate tolerance and had not been reported or applied in rice breeding. Collectively, we developed a novel dual base editor which will be valuable for artificial evolution of important genes in crops. And the novel glyphosate-tolerant rice germplasm generated in this study will benefit weeds management in rice paddy fields.
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